| 薄 海,李 玲,段富强,朱 江.低氧联合运动对大鼠骨骼肌线粒体自噬的影响[J].中国康复医学杂志,2014,29(10):908~912 |
| 低氧联合运动对大鼠骨骼肌线粒体自噬的影响 点此下载全文 |
| 薄 海 李 玲 段富强 朱 江 |
| 天津武警后勤学院军事训练医学教研室，天津市，300162 |
| 基金项目：国家自然科学基金青年基金项目（31200894），天津市应用基础及前沿技术研究计划青年基金项目（12JCQNJC07900） |
| DOI： |
| 摘要点击次数: 1834 |
| 全文下载次数: 1882 |
| 摘要： |
| 摘要
目的：观察慢性低氧及低氧联合运动对骨骼肌线粒体自噬的影响，并探讨低氧诱导因子-1α（HIF-1α）在其中的作用。
方法：56只健康雄性SD大鼠随机分为：常氧对照组（NC）、低氧对照组（HC）、低氧联合运动组（HT, n=8）、低氧+二甲基亚砜（DMSO）组（HC+D）、低氧+HIF-1α抑制剂YC-1组（HC+Y）、低氧联合运动+DMSO组（HT+D）和低氧联合运动+HIF-1α抑制剂YC-1组（HT+Y）。低氧干预为常压低氧帐篷，模拟11.3%氧浓度。运动干预为低氧帐篷内53% VO2max跑台训练，1h/d。HIF-1α抑制剂组采用YC-1腹腔注射，4mg/kg，1次/d。DMSO组注射等体积1%DMSO。上述干预均持续4周。
结果：HC组与NC组比较，线粒体膜电位、ATP合成活力显著降低（P<0.05），活性氧族（ROS）生成速率、PINK1、Parkin、Bnip3和HIF-1α蛋白表达显著升高（P<0.05）。HT组与HC组比较，线粒体膜电位、ATP合成活力、Parkin、Bnip3和HIF-1α表达显著升高（P<0.05），ROS生成速率和PINK1表达显著降低（P<0.05）。YC-1干预HC和HT组，均造成线粒体膜电位、ATP合成活力、Bnip3和HIF-1α表达显著降低（P<0.05），ROS生成速率和PINK1表达显著升高（P<0.05）。
结论：低氧联合运动可通过HIF-1α途径促进了低氧诱导的Bnip3介导的线粒体自噬保护机制，从而提高骨骼肌线粒体的质量。 |
| 关键词：低氧 运动训练 骨骼肌 线粒体自噬 低氧诱导因子α |
| Effects of hypoxia combined with exercise training on mitochondrial autophagy in skeletal muscle of rats Download Fulltext |
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| Logistics College of Chinese People's Armed Police Force, Tianjin, 300162 |
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| Abstract: |
| Abstract
Objective: To observe the effects of chronic hypoxia alone and hypoxia combined with exercise training on mitochondrial autophagy, and to identify the roles of hypoxia-inducible factor-1α (HIF-1α).
Method: A total 56 health male Sprague-Dawley rats were randomly divided into seven groups: normoxia control group (NC), hypoxia control group (HC), hypoxia combined training group (HT), dimethyl sulfoxide(DMSO) injection and hypoxia control group (HC+D), inhibitor of HIF-1α YC-1 injection and hypoxia control group (HC+Y), DMSO injection and hypoxia training group (HT+D), and YC-1 injection and hypoxia training group (HT+Y). The hypoxia rats were subjected to hypoxia exposure in normobaric hypoxic tent. The exercise training rats were administered exercise on a motor-driven rodent treadmill in normobaric hypoxic tent. Rats in HIF-1α inhibitor YC-1 groups received peritoneal injection of the YC-1 DMSO solution at 4mg/kg body weight once a day. Rats in DMSO groups received equivolume injections of the 1% DMSO solution. All interventions were administered for continuous 4 weeks.
Result: Comparing with NC group, in HC group, mitochondrial membrane potential and ATP synthesis activity showed dramatic decrease (P<0.05), and reactive oxygen species(ROS) generation, PINK1, Parkin, Bnip3, HIF-1α protein expression elevated significantly (P<0.05). Comparing with HC group in HT group, mitochondrial membrane potential, ATP synthesis activity, Parkin, Bnip3, HIF-1α protein expressions improved significantly (P<0.05), and ROS generation, PINK1 protein expression markedly decreased (P<0.05). Both in HC and HT rats, YC-1 administration significantly depressed mitochondrial membrane potential, ATP synthesis activity, Bnip3, HIF-1α protein expression (P<0.05), and markedly elevated ROS generation, PINK1 protein expression (P<0.05).
Conclusion: Hypoxia combined with exercise training could significantly promote hypoxia stress induced mitochondrial autophagy, and then elevated mitochondrial quality. HIF-1α-mediated expression of BNIP3 played an important role in this induction of mitochondrial autophagy. |
| Keywords:hypoxia exercise training skeletal muscle mitochondrial autophagy hypoxia-inducible factor-1α |
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