| 柯晓华,葛杜鹃,王文春,庞日朝,袁 青,秦慧慧,李 蓓,刘素贞,刘建成,崔银洁,张安仁.运动点电针对急性脊髓损伤大鼠半胱氨酸天冬氨酸蛋白酶3和降钙素基因相关肽表达的影响[J].中国康复医学杂志,2014,29(10):913~917 |
| 运动点电针对急性脊髓损伤大鼠半胱氨酸天冬氨酸蛋白酶3和降钙素基因相关肽表达的影响 点此下载全文 |
| 柯晓华 葛杜鹃 王文春 庞日朝 袁 青 秦慧慧 李 蓓 刘素贞 刘建成 崔银洁 张安仁 |
| 成都军区总医院康复医学科，成都，610083 |
| 基金项目：全军医学科技“十二五”面上项目（CWS11J284）；成都军区医学科学技术“十二五”计划重点项目（B12016）；成都军区总医院院管课题（2011YG-B14；2013YG-A009） |
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| 摘要： |
| 摘要
目的：探讨运动点电针对急性脊髓损伤大鼠半胱氨酸天冬氨酸蛋白酶3（caspase-3）和降钙素基因相关肽（CGRP）表达的影响。
方法：将60只SD大鼠随机分为4组，每组15只。A组：运动点电针组；B组：非运动点电针组；C组：模型组；D组：假手术组。采用改良的Allen法建立大鼠急性脊髓损伤模型。A、B组分别于术后立即开始下肢运动点和非运动点进行电针治疗；C、D组不做任何治疗。各组大鼠分别于1、4、7d行BBB评分，通过免疫组织化学方法（IHC）观察各组caspase-3和CGRP阳性细胞的表达情况。
结果：60只SD大鼠均进入结果分析。BBB评分结果显示：A组评分明显高于B、C组（P＜0.05）；免疫组化结果显示：①caspase-3的表达：损伤后各组均可见阳性细胞表达，D组仅见少量的阳性细胞。其中A、B组阳性细胞表达明显低于C组（P＜0.05）；而A组的表达又明显低于B组（P＜0.05）；②CGRP的表达：各组均可见阳性细胞表达，A、B组明显高于C组（P＜0.05），而A组又明显高于B组（P＜0.05）。
结论：运动点电针可抑制caspase-3和促进CGRP的表达，可能有助于脊髓功能的恢复。 |
| 关键词：脊髓损伤 电针 运动点 半胱氨酸天冬氨酸蛋白酶3 降钙素基因相关肽 |
| The impact of electroacupuncture at motor points on caspase-3 and calcitonin gene related peptide expression of rats with acute spinal cord injury Download Fulltext |
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| Department of Rehabilitation Medicine，General Hospital of Chengdu Military Region of PLA，Chengdu, 610083 |
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| Abstract: |
| Abstract
Objective: To discuss the impact of electroacupuncture at motor points on caspase-3 and calcitonin gene related peptide（CGRP） expressions of rats with acute spinal cord injury.
Method: Sixty SD rats with spinal cord injury were randomly divided into four groups, 15 rats in each group:group A: electroacupuncture at motor points; group B: electroacupuncture at non-motor points; group C: model group; group D: sham-operated group. Modified Allen's method was used to establish rats model of acute spinal cord injury. Rats in groups A and B acupuncture therapy were administered at motor points and non-motor points immediately after spinal cord injury. Rats in groups C and D received no treatment. At day 1,4 and 7, BBB(Basso-Beattie-Bresnahan) scores were assessed in each group,Then the expressions of caspase-3 and CGRP positive cells of rats after acute spinal cord injury in each group were observed by immunohistochemistry method.
Result: BBB scores：In group A BBB scores were significantly higher than that in groups B and C(P<0.05). Immunohistochemical results：①Caspase-3: The expressions of positive cells emerged in all groups after injury, in group D only small amount of positive cells emerged.The expressions of positive cells in groups A and B were significantly lower than that in group C (P<0.05),and the expression of positive cells in group A was lower than that in group B (P<0.05).②CGRP：The expressions of positive cells emerged in all groups. The expressions of positive cells in groups A and B were significantly higher than that in group C(P<0.05), and the expression of positive cells in group A was higher than that in group B (P<0.05).
Conclusion: Electroacupuncture at motor points can suppress the expression of caspase-3 and promote the expression of CGRP and would be helpful for the spinal cord function recovery. |
| Keywords:spinal cord injury electroacupuncture motor point caspase-3 calcitonin generelated peptide |
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