| 沈夏锋,于惠贤,吴军发,张宇玲,田 闪,荣积峰,路微波,胡永善,吴 毅.早期跑台训练在改善颅脑外伤大鼠认知功能中的应用[J].中国康复医学杂志,2015,(2):112~116 |
| 早期跑台训练在改善颅脑外伤大鼠认知功能中的应用 点此下载全文 |
| 沈夏锋 于惠贤 吴军发 张宇玲 田 闪 荣积峰 路微波 胡永善 吴 毅 |
上海第一康复医院(筹)
，上海杨浦区老年医院,200090 |
| 基金项目：上海市杨浦区卫生局“名医师工程”课题；上海市科委生物医学重点项目（13411951000） |
| DOI： |
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| 摘要： |
| 摘要
目的：观察早期运动对中重度颅脑外伤（TBI）大鼠认知功能的影响。
方法：成年雄性SD大鼠30只，随机分为运动组、对照组和假手术组。制作中重度控制性皮质损伤（CCI）颅脑外伤模型，运动组大鼠在术后1天进行电动跑台运动训练，30min/d，1次/d，为期2周，训练结束后休息1周；术后第21—25天行Morris水迷宫(MWM)检查，评估大鼠认知功能的变化；术后第26天行Western blot检查海马区脑源性神经营养因子（BDNF）表达。
结果：运动组术后第21—24天平台搜索的潜伏期较对照组缩短，术后第25天颅脑外伤后运动组的象限游泳路程占总路程的百分值（29.1±0.02）大于对照组（23.10±0.02）(P<0.05)；运动组和对照组大鼠损伤对侧皮质内BDNF蛋白表达分别为（0.94±0.10）和（0.65±0.03），表明运动上调损伤对侧的海马区BDNF的表达。
结论：早期进行适宜强度的运动训练治疗能改善颅脑外伤大鼠认知功能和上调海马BDNF的表达。 |
| 关键词：颅脑损伤 跑台训练 认知功能 海马 脑源性神经营养因子 |
| Early treadmill exercise in improving cognitive functional deficit after traumatic brain injury in rats Download Fulltext |
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| Dept. of Rehabilitation Medicine,The Shanghai No.1 Rehabilitation Hospital,Shanghai,200090 |
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| Abstract: |
| Abstract
Objective: To determine the effect of early treadmill exercise on cognitive function of rats following traumatic brain injury(TBI）.
Method: Thirty rats were randomly divided into an exercise group(n=10), a control group(n=10) or a sham group(n=10). Rats underwent surgery to induce either a sham injury or a controlled cortical impact (CCI) injury. Rats in exercise group were forced to administers treadmill exercise for 14 days. The Morris water maze(MWM) task began at 21 days post-surgery and lasted for consecutive 4 days. Rats were sacrificed at day 26 for determining brain-derived neurotrophic factor (BDNF) protein expression in hippocampus.
Result: The rats in the exercise group had a shorter latency to find the platform in MWM test compared with control group rats (P<0.05). In exercise group rats travelled a longer distance which possesed a larger percent of total distance(29.07±0.02), compared to that in control group (23.10±0.02)(P<0.05). The BDNF protein levels in contralateral hippocampus increased in the exercise group compared with the control group,(0.944±0.095)vs (0.65±0.03), respectively.
Conclusion: Treadmill exercise soon after severe TBI can contribute to the alleviation of TBI-induced cognitive deficits and the up-regulation of hippocampal BDNF expression. |
| Keywords:Dept. of Rehabilitation Medicine,The Shanghai No.1 Rehabilitation Hospital,Shanghai,200090 |
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