| 钟颖君,王 亮,魏天琪,冯 珍.正中神经电刺激对脑外伤后昏迷大鼠前额叶皮质及下丘脑Orexin-A及其受体OX1R表达变化的影响[J].中国康复医学杂志,2015,(7):640~644 |
| 正中神经电刺激对脑外伤后昏迷大鼠前额叶皮质及下丘脑Orexin-A及其受体OX1R表达变化的影响 点此下载全文 |
| 钟颖君 王 亮 魏天琪 冯 珍 |
| 南昌大学第一附属医院康复医学科，南昌，330006 |
| 基金项目：国家自然科学基金资助项目（81260295）；江西省自然科学基金资助项目（20132BAB205063） |
| DOI： |
| 摘要点击次数: 2007 |
| 全文下载次数: 2030 |
| 摘要： |
| 摘要
目的：探讨正中神经电刺激（MNES）对脑外伤（TBI）昏迷大鼠的促醒作用及其可能机制。
方法：将SD大鼠分为3组：空白对照组、假刺激组和刺激组。应用MNES治疗脑外伤后昏迷大鼠，观察其行为学变化，并用免疫组织化学、Western-blot、ELISA技术检测各组大鼠前额叶皮质（PFC）和下丘脑组织的Orexin-A及OX1R含量。
结果：刺激组30只大鼠中21只出现翻正反射，假刺激组30只中7只出现翻正反射；将3组的Orexin-A及OX1R含量进行两两比较，发现刺激组Orexin-A及受体水平高于其他两组，差异有显著性意义（P＜0.05）。
结论：MNES可作为脑外伤后昏迷的有效促醒手段，其机制可能与PFC及下丘脑部位Orexin-A及OX1R水平上调有关。 |
| 关键词：正中神经电刺激 脑外伤 促醒 昏迷 Orexin-A OX1R |
| Wake-promoting action of median nerve electrical stimulation in traumatic brain injury-coma： a mechanical study of expressions of Orexin-A and Orexin receptor 1 in prefrontal cortex and hypothalamic area Download Fulltext |
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| Department of Rehabilitation Medicine, the First Affiliated Hospital of Nanchang University, Medical School of Nanchang University, Nanchang, 330006 |
| Fund Project: |
| Abstract: |
| Abstract
Objective： To investigate the wake-promoting action and potential mechanisms of median nerve electrical stimulation(MNES) in traumatic brain injury(TBI)-coma rats.
Method： Ninety rats were divided into three groups： control, sham-stimulated (TBI) and stimulated (TBI+MNES) group. MNES was applied to rats after TBI-coma models were established. Then, behaviors of rats were observed and Orexin-A and OX1R in prefrontal cortex(PFC) and hypothalamus area were examined via immunohistochemistry, Western-blotting and ELISA techniques.
Result： In stimulated group, 21 rats exhibited righting reflex and in TBI group, 7 rats had the same responses. In stimulated group, both in PFC and hypothalamus area, Orexin-A and OX1R levels were higher than those in other groups (P＜0.05).
Conclusion： MNES might be an effective wake-promoting way for TBI-coma. The mechanism might be due to upregulating the excitatory hypothalamic neuropeptide Orexin-A and OX1R expression in PFC and hypothalamic area. |
| Keywords:median nerve electrical stimulation traumatic brain injury wake-promoting coma Orexin-A OX1R |
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