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李春燕,彭洪卫,李晓洁,林如辉,陶 静,陈立典.电针对脑缺血再灌注大鼠学习记忆行为及海马区α7烟碱型乙酰胆碱受体的影响[J].中国康复医学杂志,2016,(2):167~171
电针对脑缺血再灌注大鼠学习记忆行为及海马区α7烟碱型乙酰胆碱受体的影响    点此下载全文
李春燕  彭洪卫  李晓洁  林如辉  陶 静  陈立典
福建中医药大学康复医学院,福州,350108
基金项目:福建省康复技术协同创新中心资助项目(x2012004-协同)
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摘要:
      摘要 目的:探讨电针对脑缺血再灌注大鼠学习记忆行为及海马区α7烟碱型乙酰胆碱受体(alpha7 nicotinic acetylcholine receptor, α7nAChR)的影响。 方法:将45只雄性SD大鼠按随机数字表法分为假手术组、模型组和电针组,每组均15只。模型组和电针组均参照Longa改良线栓法制备大鼠局灶性脑缺血再灌注模型。电针组电针神庭、百会穴,共7d,每次30min。采用Morris水迷宫实验观察大鼠的学习记忆功能;HE染色法观察大鼠海马神经元细胞结构变化;免疫组织化学染色法观测大鼠海马区α7nAchR免疫阳性细胞的表达;Western blot法检测大鼠海马区α7nAChR蛋白的表达。 结果:各组大鼠游泳速度未见显著性差异(P>0.05);模型组与假手术组相比大鼠逃避潜伏期明显延长(P<0.01),跨越平台次数明显减少(P<0.01);电针组与模型组相比大鼠逃避潜伏期明显缩短(P<0.01),跨越平台次数明显增加(P<0.01)。与假手术组相比,模型组海马区CA1区α7nAChR免疫阳性细胞表达及整个海马区α7nAChR蛋白的表达降低(P<0.01),海马神经元细胞损伤加重;与模型组相比,电针上调海马区CA1区α7nAChR免疫阳性细胞表达及整个海马区α7nAChR蛋白的表达(P<0.01),同时降低海马神经元细胞的损伤。 结论:电针能改善脑缺血再灌注损伤大鼠的学习记忆能力,保护神经元细胞,其机制可能与上调海马区α7nAChR表达有关。
关键词:电针  脑缺血  学习记忆  α7烟碱型乙酰胆碱受体
Effects of electroacupuncture on learning and memory ability and the expression of α7nAChR in rats with cerebral ischemia reperfusion injury    Download Fulltext
College of Rehabilitation Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, 350108
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Abstract:
      Abstract Objective: To explore the effects of electroacupuncture on learning and memory ability and the expression of α7nAChR in rats with focal cerebral ischemia reperfusion injury. Method: Forty-five male SD rats were randomly divided into sham group (n=15), model group (n=15) and electroacupuncture group (n=15). The latter 2 groups were performed with middle cerebral artery occlusion for 2h and reperfusion. The electroacupuncture group received electroacupuncture treatment at Shenting (DU24) and Baihui (DU20) for 30min, once a day for 7 days. Learning and memory of the rats was tested by Morris water maze. The morphological changes of hippocampus neurons were observed by HE staining. The expression level of α7nAChR was determined with immunohistochemical staining and Western blot. Result: There were no significant differences in the swimming speed among the control, model group and electroacupuncture group(P>0.05). The rats in the model group demonstrated longer latency time to reach the hidden platform(P<0.01) and passed the platform position fewer times in the water maze tests(P<0.01) than the control group. In the electroacupuncture group, the rats significantly shorten the latency time(P<0.01) and increased the frequency of passing the platform(P<0.01) compared with the model group. And compared with the model group, electroacupuncture reduced pathological damage of neurons, increased α7nAChR immune positive cells in the CA1 and the expression of α7nAChR in hippocampus (P<0.01). Conclusion: Electroacupuncture was helpful for improving learning and memory ability in rats of cerebral ischemia/reperfusion injury, which may associate with increasing the expression of α7nAChR in hippocampus.
Keywords:electroacupuncture  cerebral ischemia  learning and memory  α7 nicotinic acetylcholine receptor
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