| 邓德万,林建平,陈少清,夏 梦,王诗忠.电针委中穴对腰椎退变模型大鼠纤维环细胞caspase3、XIAP蛋白表达的影响[J].中国康复医学杂志,2016,(4):394~398 |
| 电针委中穴对腰椎退变模型大鼠纤维环细胞caspase3、XIAP蛋白表达的影响 点此下载全文 |
| 邓德万 林建平 陈少清 夏 梦 王诗忠 |
| 福建中医药大学,福建福州，350122 |
| 基金项目：国家自然科学基金资助项目(81303017)；福建省自然科学基金（2014J01346）；福建省教育厅A类科技项目（JA13163） |
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| 摘要
目的：观察电针委中穴对腰椎退变模型大鼠纤维环细胞凋亡及caspase3和XIAP表达的影响。
方法：将40只雄性SD大鼠按随机数字表法分为假手术组、模型组、电针委中穴组、电针非穴组。采用纤维环穿刺法制作腰椎退变模型。于造模1个月后行电针治疗，电针委中穴组取“委中”穴，电针非穴组取委中内侧0.5cm，均每日1次，每次20min，连续治疗4周。治疗结束后取材，运用TUNEL法检测纤维环细胞凋亡，免疫组化检测（caspase3）和XIAP表达。
结果：假手术组偶见TUNEL细胞，caspase3低表达，XIAP高表达；模型组纤维环细胞TUENL阳性细胞和caspase3表达较假手术组显著增加（P<0.01），XIAP表达低于假手术组（P<0.01）；与模型组比较，电针委中穴组TUNEL阳性细胞和caspase3表达均明显减少（P<0.01），XIAP表达增加（P<0.01）；与电针委中穴组比较，电针非穴组纤维环细胞TUENL阳性细胞和caspase3表达较高（P<0.05），XIAP蛋白表达减少（P<0.05）。
结论：电针可延缓椎间盘退变，其机制可能与调节纤维环细胞caspase3和XIAP表达，抑制纤维环细胞凋亡有关。 |
| 关键词：委中穴 椎间盘退变 纤维环 caspase3 XIAP |
| Effects of electro-acupuncture of “Weizhong” point on the expressions of caspase3 and XIAP in rat with lumbar disc degeneration Download Fulltext |
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| Fujian University of Traditional Chinese Medicine, Fuzhou,350122 |
| Fund Project: |
| Abstract: |
| Abstract
Objective：To observe the effects of electro-acupuncture (EA) of “Weizhong”(BL40) point on the expressions of caspase3 and XIAP in rat with lumbar disc degeneration so as to explore the underlying mechanism.
Method：Forty male adult SD rats were randomly divided into sham-operated, model, EA-BL40 and non-point groups. The rat model of lumbar disc degeneration set up by puncturing the annulus fibrosis with needles with defined gauges. Following modeling, EA was performed at BL40 for 20 min, once daily for 4 weeks, with one day's interval between two weeks. The non-point was located 0.5cm lateral to the BL40 and stimulated by EA similar to EA-BL40. At the end of the experiment, lumbar disc tissues were taken out for TUNEL and detecting the expression of caspase3 and XIAP with immunohistochemistry.
Result：Apoptotic cells and caspase3 in annulus fibrosus of model group was higher than that of sham-operated group（P<0.01）,and expression of XIAP decreased in group of model（P<0.01）. In comparison with the model group, expression of apoptotic cells and caspase3 decreased in group of EA-BL40（P<0.01）, and the expression of XIAP increased significantly in EA-BL40 group（P<0.01）. Compared with the EA-BL40 group, expression of apoptotic cells and caspase3 increased in non-point group（P<0.05）, and expression of XIAP decreased significantly（P<0.05）.
Conclusion: EA could regulate the expression of caspase3 and XIAP to delay disc degeneration. |
| Keywords:Weizhong point intervebral disc degeneration annulus fibrous caspase3 XIAP |
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