| 李亚丽,刘合玉,夏 彬.减重平板训练联合甲基强的松龙对脊髓损伤大鼠运动功能和脑源性神经营养因子及其受体TrkB表达的影响[J].中国康复医学杂志,2016,(8):852~856 |
| 减重平板训练联合甲基强的松龙对脊髓损伤大鼠运动功能和脑源性神经营养因子及其受体TrkB表达的影响 点此下载全文 |
| 李亚丽 刘合玉 夏 彬 |
| 郑州大学第二附属医院神经康复科，450014 |
| 基金项目： |
| DOI： |
| 摘要点击次数: 1236 |
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| 摘要
目的：观察减重平板训练（BWSTT）和甲基强的松龙（MP）联合应用对脊髓损伤大鼠运动功能及脑源性神经营养因子（BDNF）及其酪氨酸激酶受体B（TrkB）表达的影响，为临床治疗脊髓损伤提供理论依据。
方法：清洁级成年雄性SD大鼠48只，按随机分配法，分为损伤对照组（n=12）、BWSTT组（n=12）、MP组（n=12）、联合治疗组（n=12）。采用改良Allen撞击法制作T10不完全性脊髓损伤模型。损伤对照组损伤后不做处理，减重平板组于损伤后1周平板训练，MP组损伤后给予大剂量甲基强的松龙琥珀酸钠冲击治疗，联合治疗组损伤后给予MP治疗联合平板训练。每组分别在损伤前、损伤后1周、2周、3周、4周、5周时采用BBB评分进行运动功能评定。于训练结束时取T12—L2节段脊髓，采用免疫组化法结合光密度分析法观察BDNF及其受体TrkB的表达。
结果：①每组大鼠损伤后5周时运动功能评分均较本组1周时评分显著增高（P＜0.01）。②从损伤3周后，与损伤对照组比较，其余3组大鼠运动功能评分明显增高，且联合治疗组显著高于减重平板组和MP组，5周时，减重平板组评分高于MP组，差异具有显著性（P＜0.05）。③免疫组化结果与对照组比较，减重平板组、MP组、联合组大鼠脊髓组织BDNF及其受体Trkb表达明显上升，差异有显著性（P＜0.05），联合组BDNF及Trkb表达高于单纯平板组和MP组，差异具有显著性（P＜0.05）。
结论：减重平板训练联合应用甲基强的松龙比单纯减重平板训练和MP对SCI大鼠运动功能及神经营养因子BDNF及其受体TrkB的表达有更明显的促进作用。 |
| 关键词：减重平板训练 甲基强的松龙 脊髓损伤 运动功能 脑源性神经营养因子 |
| Effects of body weight support treadmill training combined with methylprednisolone on motor function and expressions of brain derived neurotrophic factor and TrkB in injured spinal cord of rats Download Fulltext |
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| Department of Neurological Rehabilitation Second Hospital of Zhengzhou University，Zhengzhou，450014 |
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| Abstract: |
| Abstract
Objective: To explore effects of body weight support treadmill training(BWSTT) combined with high-dose methylprednisolone(MP) on motor function and expressions of brain-derived neurotrophic factor(BDNF) and tyrosine kinase receptor B(TrkB) in rats with spinal cord injury, provide theoretical basis for clinical treatment of spinal cord injury.
Method: Forty-eight adult male SD rats of clean level were randomly divided into damage control group (n=12), BWSTT group (n=12), MP group (n=12), BWSTT combined with high-dose MP group (n=12). An animal model of incomplete spinal cord injury was established by using modified Allen's method at T10 level of spinal cord. SCI group had no processing after injury, BWSTT group was trained by BWSTT for 4 weeks, MP group was treated by MP and combined group was treated by MP combined with training by BWSTT. Motion function of every group was evaluated by using BBB scale before injury and at the 1st, 2nd, 3rd, 4th, 5th week post injury. The expression level of BDNF and TrkB in spinal cords were detected by immunohistochemical method.
Result: ① Five weeks after injury, BBB scores of each group were significantly higher than 1 week after injury(P＜0.01). ② Since the 3rd week after injury, BBB scores in other three groups were higher than that in damage control group; the scores of combined group were higher than that in BWSTT and MP groups. Five weeks after injury, BBB scores in BWSTT group were higher than that in MP group (P＜0.05). ③Compared with SCI group, the expression levels of BDNF and TrkB in combined group increased significantly(P＜0.05), and were significantly higher than BWSTT or MP alone(P＜0.05).
Conclusion: BWSTT combined with MP could more effectively promote the recovery of motor function and the expression levels of BDNF and TrkB in SCI rats than BWSTT and MP alone. |
| Keywords:body weight support treadmill training methylprednisolone spinal cord injury motor function brain-derived neurotrophic factor |
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