| 熊伟平,夏 志.有氧运动促进老年前期小鼠快缩型骨骼肌蛋白质合成代谢的研究[J].中国康复医学杂志,2016,(12):1311~1317 |
| 有氧运动促进老年前期小鼠快缩型骨骼肌蛋白质合成代谢的研究 点此下载全文 |
| 熊伟平 夏 志 |
| 保山学院体育学院，云南保山，678000 |
| 基金项目：云南省教育厅科学研究基金（2012Y259）；江西省自然科学基金青年项目（20142BAB215048） |
| DOI： |
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| 摘要
目的：探讨中等强度有氧运动对老年前期小鼠快缩型骨骼肌蛋白质合成代谢的保护作用及其可能性机制。
方法：20只13月龄雄性CD-1小鼠根据体重随机纳入对照组（C组）与有氧运动干预组（E组），运动组以自体重3%的负荷进行8周游泳训练，每日训练1次，每次45min，每周训练6天。末次运动后24h处死小鼠（禁食17h），采用便携式血糖仪检测血糖，氨基酸分析仪检测血浆游离氨基酸谱，酶联免疫法检测血清胰岛素，BCA法进行蛋白定量，免疫印迹法检测mTORSer2448、4E-BP1Thr37/46与p70S6KThr389磷酸化表达以及MHCⅡ蛋白表达。
结果：与对照组（C组）小鼠相比，有氧运动干预组（E组）小鼠血浆游离氨基酸水平表现出下降趋势，其中亮氨酸、谷氨酸与甘氨酸含量下降显著（P<0.01）。腓肠白肌蛋白质总量、mTORSer2448、4E-BP1Thr37/46、p70S6KThr389磷酸化表达与MHCⅡ蛋白表达均明显升高，有显著性意义（P<0.01）。
结论：中等强度有氧运动可以促进老年前期小鼠快缩型骨骼肌蛋白质合成，可能具有削弱衰老性肌肉萎缩发生发展的潜力，其作用机制可能涉及肌细胞内mTOR/p70S6K信号通路的活化。 |
| 关键词：有氧运动 衰老 快缩型骨骼肌 蛋白质合成 |
| Aerobic exercise promotes protein synthesis in fast-twitch skeletal muscle of pre-senile mice Download Fulltext |
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| Baoshan Collage Sports Institute, Baoshan, Yunnan, 678000 |
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| Abstract: |
| Abstract
Objective: To investigate the protective effect and possible mechanism of moderate intensity aerobic exercise on protein synthesis in fast-twitch skeletal muscle of pre-senile mice.
Method: Twenty thirteen-month-old male CD-1 mice were randomly divided into normal control group and aerobic exercise group by body weight. Mice received 8 weeks moderate swimming exercise training with 3% body weight workload, 45min/day, +6 days/w. Twenty four hours after the final exercise bout (17 h fasting), mice were all sacrificed. The amino acids analyzer was used to determine the profile of plasma free amino acids. The blood glucose was determined by the blood glucose meter. The serum insulin content was detected by enzyme linked immunosorbent assay. The total protein content in white gastrocnemious muscles was detected by BCA protein assay. And at the same time, the phosphorylation rate of mTORSer2448,4E-BP1Thr37/46,p70S6KThr389 and the expression of MHCⅡ protein were determined by using the Western blotting method.
Result: When compared with mice in control group, mice received swimming exercise training showed a declining trend of plasma free amino acids and the content of leucine, glutamic acid and glycine decreased significantly(P<0.01), while the total protein, phosphorylation rate of mTORSer2448, 4E-BP1Thr37/46, p70S6KThr389 and the protein expression of MHCⅡ were all elevated with statistical significance (P<0.01).
Conclusion: Moderate aerobic exercise promotes protein synthesis in white gastrocnemious muscles of pre-senile mice, and the mTOR/p70S6K pathway may contribute to this effect. These results suggest that moderate aerobic exercise may play a potential role in attenuating the occurrence and development of sarcopenia. |
| Keywords:aerobic exercise aging fast-twitch skeletal muscle protein synthesis |
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