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孔明涯,陆 晓,林 松,赵 彦,林爱翠.生理性缺血训练对动脉粥样硬化进程中兔血管内皮的影响[J].中国康复医学杂志,2017,(1):10~14
生理性缺血训练对动脉粥样硬化进程中兔血管内皮的影响    点此下载全文
孔明涯  陆 晓  林 松  赵 彦  林爱翠
南京林业大学体育部,210037
基金项目:国家自然科学基金项目(81101456);南京市医学科技发展资金项目(QRX11248,YKK14097);江苏省高校自然科学基金项目(14KJB320002);南京医科大学科技发展基金项目(2013NJMU077)
DOI:
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摘要:
      摘要 目的:研究动脉粥样硬化早期进行生理性缺血训练对斑块形成的影响。 方法:成年新西兰白兔24只,随机分为3组,每组8只,分别为对照组、高脂组和训练组。对照组采用普通饮食,其余两组采用高脂饮食,其中训练组左下肢用止血带结扎的方式进行生理性缺血训练3min/次,3次/d,5d/周,另外两组安静笼养。实验周期为4周。 结果:4周实验后,兔主动脉油红-O染色结果显示,高脂组主动脉血管壁上动脉粥样硬化斑块面积占1.84±0.83%,而对照组和训练组却没有动脉粥样硬化斑块形成。血管横切面HE染色显示高脂组兔主动脉内膜上已经形成泡沫细胞,而对照组和训练组兔的主动脉内膜光滑,无泡沫细胞形成。实验前三组兔血液VEGF含量组间差异无显著性意义(对照组:8.35±0.37pg/ml,高脂组:8.27±0.55pg/ml,训练组:8.63±0.36pg/ml,P>0.05),实验后三组兔血液VEGF含量均高于实验前,但组间差异无显著性意义(对照组:16.93±0.89pg/ml,高脂组:14.39±0.97pg/ml,训练组:14.36±0.73pg/ml,P>0.05)。实验前三组兔血液NO含量组间差异无显著性意义(对照组:0.888±0.23μmol/L,高脂组:0.421±0.09μmol/L,训练组:0.529±0.134μmol/L,P>0.05),实验后三组兔血液NO含量均高于实验前,组间差异具有显著性意义(P<0.01),训练组(2.18±0.144μmol/L)和高脂组(1.82±0.078μmol/L)均显著高于对照组(1.27±0.167μmol/L)。实验前三组兔血液EPCs含量组间差异无显著性意义(对照组:7.25±0.86个/每105个淋巴细胞,高脂组:7.5±0.6个/每105个淋巴细胞,训练组:8.87±0.61个/每105个淋巴细胞,P>0.05),实验后训练组EPCs含量(12.75±0.94个/每105个淋巴细胞)高于高脂组(8.25±0.73个/每105个淋巴细胞)和对照组(8.25±0.73个/每105个淋巴细胞)且组间差异具有显著性意义(P<0.01)。 结论:在动脉粥样硬化形成过程中,生理性缺血训练可以促进机体产生血管内皮相关因子,保护血管内皮功能,减缓粥样硬化斑块形成速度。
关键词:生理性缺血训练  动脉粥样硬化  血管内皮生长因子  血管内皮功能障碍
Effects of physiological ischemia training on rabbits’ vascular endothelium during atherosclerotic plaque formation process    Download Fulltext
Dept. of Physical Education, Nanjing Forestry University, Nanjing, 210037
Fund Project:
Abstract:
      Abstract Objective: To investigate the effect of physiological ischemia training on atherosclerotic plaque formation at the early stage of atherosclerosis. Method: Twenty-four adult New Zealand rabbits were randomly assigned into three groups: control group, high-fat diet group and training group. Rabbits in control group were feed with normal rabbit food, while animals in high-fat diet group and training group were feed with high fat food. Rabbits in training group experienced physiological ischemia training with 3mins/time, 3 times/day, 5days/week. The total experiment was lasted for 4 weeks. Result: At the end of experiment, the result of oil-red O dyed aorta showed 1.84±0.83% atherosclerotic plaque on the aortic vascular wall in high-fat diet group, but nothing in control group and training group. HE staining of vascular cross section showed foam cell formation, but no foam cell formation in control group and training group. At the beginning of experiment, there was no significant difference (P>0.05) of VEGF concentration among control group (8.35±0.37 pg/ml), high-fat diet group (8.27±0.55 pg/ml) and training group (8.63±0.36 pg/ml). It increased for control group (16.93±0.89 pg/ml), high-fat diet group (14.39±0.97 pg/ml) and training group (14.36±0.73 pg/ml) at the end of experiment. However, there was no significant difference among the three groups(P>0.05). At the beginning of experiment, there was no significant difference(P>0.05) of nitric oxide among control group (0.888±0.23μmol/L), high-fat diet group (0.421±0.09μmol/L) and training group (0.529±0.134μmol/L). At the end of experiment, nitric oxide concentration in high-fat diet group (2.18±0.144μmol/L) and training group (1.82±0.078μmol/L) increased significantly (P<0.01) when compare with that in control group (1.27±0.167μmol/L). There’s no significant difference (P>0.05) of EPCs concentration among control group (7.25±0.86/105 lymphocyte), high-fat diet group (7.5±0.6/105 lymphocyte) and training group (8.87±0.61/105 lymphocyte) at the beginning of experiment. At the end of experiment, EPCs concentration in training group (12.75±0.94/105 lymphocyte) was significantly higher than that in high-fat diet group (8.25±0.73/105 lymphocyte) and control group (8.25±0.73/105 lymphocyte)(P<0.01). Conclusion: During the formation of atherosclerosis, physiological ischemia training can promote the production of the vascular endothelial related factors that protect vascular endothelial function and slow down the formation of atherosclerosis plaque.
Keywords:physiological ischemia training  atherosclerosis  vascular endothelial growth factor  vascular endothelial dysfunction
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