| 陈 琴,黄菲菲,董晓阳,冯 珍.迷走神经电刺激对脑外伤后昏迷大鼠前额叶皮质去甲肾上腺素α1受体表达变化的影响[J].中国康复医学杂志,2017,(1):28~32 |
| 迷走神经电刺激对脑外伤后昏迷大鼠前额叶皮质去甲肾上腺素α1受体表达变化的影响 点此下载全文 |
| 陈 琴 黄菲菲 董晓阳 冯 珍 |
| 南昌大学第一附属医院康复医学科，南昌，330006 |
| 基金项目：国家自然科学基金资助项目（81260295） |
| DOI： |
| 摘要点击次数: 1865 |
| 全文下载次数: 1707 |
| 摘要： |
| 摘要
目的：探讨迷走神经电刺激（vagus nerve stimulation， VNS）对脑外伤（traumatic brain injury， TBI）后昏迷大鼠的促醒作用及其可能机制。
方法：健康成年SD大鼠90只，随机分为空白对照组、假刺激组和刺激组。TBI后昏迷大鼠给予VNS治疗，观察其行为学变化，并通过免疫组织化学和Western-blot技术检测各实验组大鼠前额叶皮质（prefrontal cortex， PFC）组织中去甲肾上腺素α1受体（α1R）含量。
结果：假刺激组和刺激组大鼠各30只，观察行为学变化发现，刺激组中20只出现翻正反射，假刺激组仅8只出现翻正反射；将3个实验组中α1R含量进行两两比较，发现刺激组α1R水平均显著高于其他两组（P＜0.05）。
结论：VNS可改善TBI后昏迷大鼠的意识状态水平，对促进TBI后昏迷大鼠的觉醒有积极的治疗作用，其机制可能与PFC去甲肾上腺素α1R水平上调有关。 |
| 关键词：迷走神经电刺激 脑外伤 昏迷 促醒 去甲肾上腺素α1受体 |
| Effect of vagus nerve stimulation on expression of α1-adrenergic receptor in prefrontal cortex of coma rats induced by traumatic brain injury Download Fulltext |
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| Department of Rehabilitation Medicine, The First Affiliated Hospital of Nanchang University，Jiangxi, 330006 |
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| Abstract: |
| Abstract
Objective: To investigate the wake-promoting action of vagus nerve stimulation (VNS) in traumatic brain injury (TBI) induced coma rats and its possible mechanism.
Method: Ninety healthy Sprague Dawley (SD) rats were randomly divided into 3 groups: control, sham-stimulated (TBI) and stimulated (TBI+VNS) group. VNS was applied to rats after TBI induced coma models. Then, to observe the changes in the behavior of rats and to detect the content of α1-adrenergic receptor in the prefrontal cortex (PFC) by using immunohistochemistry and Western-blotting techniques.
Result: Twenty rats in the stimulated group exhibited righting reflex and 8 rats had the same responses in TBI group. In stimulated group, the level of α1-adrenergic receptor in PFC were significantly higher than those in other groups (P＜0.05).
Conclusion: VNS can improve the level of consciousness of the rats after TBI-coma, and has a positive effect on wake-promoting action. The potential mechanism might be related to upregulating the expression of α1-adrenergic receptor in PFC. |
| Keywords:vagus nerve stimulation traumatic brain injury coma wake-promoting α1-adrenergic receptor |
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