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陈 琴,黄菲菲,董晓阳,冯 珍.迷走神经电刺激对脑外伤后昏迷大鼠前额叶皮质去甲肾上腺素α1受体表达变化的影响[J].中国康复医学杂志,2017,(1):28~32
迷走神经电刺激对脑外伤后昏迷大鼠前额叶皮质去甲肾上腺素α1受体表达变化的影响    点此下载全文
陈 琴  黄菲菲  董晓阳  冯 珍
南昌大学第一附属医院康复医学科,南昌,330006
基金项目:国家自然科学基金资助项目(81260295)
DOI:
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摘要:
      摘要 目的:探讨迷走神经电刺激(vagus nerve stimulation, VNS)对脑外伤(traumatic brain injury, TBI)后昏迷大鼠的促醒作用及其可能机制。 方法:健康成年SD大鼠90只,随机分为空白对照组、假刺激组和刺激组。TBI后昏迷大鼠给予VNS治疗,观察其行为学变化,并通过免疫组织化学和Western-blot技术检测各实验组大鼠前额叶皮质(prefrontal cortex, PFC)组织中去甲肾上腺素α1受体(α1R)含量。 结果:假刺激组和刺激组大鼠各30只,观察行为学变化发现,刺激组中20只出现翻正反射,假刺激组仅8只出现翻正反射;将3个实验组中α1R含量进行两两比较,发现刺激组α1R水平均显著高于其他两组(P<0.05)。 结论:VNS可改善TBI后昏迷大鼠的意识状态水平,对促进TBI后昏迷大鼠的觉醒有积极的治疗作用,其机制可能与PFC去甲肾上腺素α1R水平上调有关。
关键词:迷走神经电刺激  脑外伤  昏迷  促醒  去甲肾上腺素α1受体
Effect of vagus nerve stimulation on expression of α1-adrenergic receptor in prefrontal cortex of coma rats induced by traumatic brain injury    Download Fulltext
Department of Rehabilitation Medicine, The First Affiliated Hospital of Nanchang University,Jiangxi, 330006
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Abstract:
      Abstract Objective: To investigate the wake-promoting action of vagus nerve stimulation (VNS) in traumatic brain injury (TBI) induced coma rats and its possible mechanism. Method: Ninety healthy Sprague Dawley (SD) rats were randomly divided into 3 groups: control, sham-stimulated (TBI) and stimulated (TBI+VNS) group. VNS was applied to rats after TBI induced coma models. Then, to observe the changes in the behavior of rats and to detect the content of α1-adrenergic receptor in the prefrontal cortex (PFC) by using immunohistochemistry and Western-blotting techniques. Result: Twenty rats in the stimulated group exhibited righting reflex and 8 rats had the same responses in TBI group. In stimulated group, the level of α1-adrenergic receptor in PFC were significantly higher than those in other groups (P<0.05). Conclusion: VNS can improve the level of consciousness of the rats after TBI-coma, and has a positive effect on wake-promoting action. The potential mechanism might be related to upregulating the expression of α1-adrenergic receptor in PFC.
Keywords:vagus nerve stimulation  traumatic brain injury  coma  wake-promoting  α1-adrenergic receptor
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