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洪 玲,赵晓霖,陈秀云,林 诚,潘燕霞.早期运动训练用于预防高血压大鼠心功能降低及心肌肥厚的研究[J].中国康复医学杂志,2017,(2):161~166
早期运动训练用于预防高血压大鼠心功能降低及心肌肥厚的研究    点此下载全文
洪 玲  赵晓霖  陈秀云  林 诚  潘燕霞
福建医科大学医学技术与工程学院康复系,福州,350004
基金项目:国家自然科学基金资助项目(81372111);福建省自然科学基金资助项目(2014J01339)
DOI:
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摘要:
      摘要 目的:观察高血压前期运动训练对自发性高血压大鼠血压、心功能、心脏质量和心肌肾素-血管紧张素系统影响,探讨运动训练改善心功能和心肌肥厚的作用机制。 方法:5周龄雄性自发性高血压大鼠(SHR)和正常血压大鼠(WKY)随机分成安静组(S)和运动训练组(E),4组大鼠分别为:SHR-S,SHR-E,WKY-S和WKY-E,每组10只。运动组进行8周中低强度的跑台运动(60min/d,18—20m/s, 5d/周)。尾套法测定大鼠收缩压(SBP),心室插管检测左室舒张末压(LVEDP)、左室压力最大上升和下降速率(±dp/dt max)。左心室质量指数(LVMI)反映心肌肥厚程度。Real-time PCR检测左室心肌血管紧张素转换酶(ACE)和血管紧张素Ⅱ 1型(AT1)受体 mRNA表达,Western blot 分析ACE蛋白水平,ELISA法检测血管紧张素Ⅱ(AngⅡ)水平。 结果:运动前,5周龄SHR大鼠SBP与正常WKY无明显差异。运动训练结束,与WKY-S组相比,SHR-S组SBP、LVEDP和LVMI均显著升高(P<0.01),-dp/dt max则明显减慢(P<0.01)。SHR-S组左室心肌ACE、AT1 mRNA表达、ACE蛋白和AngⅡ水平均较WKY-S组明显升高(P<0.01)。SHR-E组SBP、LVEDP和LVMI较SHR-S组明显降低(P<0.01),-dp/dt max则明显高于SHR-S组(P<0.05)。SHR-E组左室心肌ACE、AT1mRNA表达、ACE蛋白和AngⅡ水平较SHR-S组均明显改善(P<0.01)。WKY-E组SBP、LVEDP、LVMI、±dp/dt max等指标与WKY-S组比较呈轻度改变,但均未达到显著性意义,WKY-E组左室心肌ACE、AT1mRNA表达、ACE蛋白和AngⅡ水平与WKY-S组无显著差别。 结论:高血压前期运动训练延缓SHR大鼠高血压进展、预防心脏舒张功能降低和心肌肥厚,其机制可能与运动训练抑制心脏过度激活的ACE-AngⅡ-AT1轴的作用有关。
关键词:高血压前期  运动训练  心功能  肾素-血管紧张素系统
Early exercise training prevents reduction of cardiac function and cardiac hypertrophy in hypertensive rats    Download Fulltext
Department of Rehabilitation Medicine of Fujian Medical University, Fuzhou, 350004
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Abstract:
      Abstract Objective:To observe the effects of exercise training at the prehypertensive stage on blood pressure, cardiac function and mass as well as renin-angtiotensin system in the left ventricle and to investigate its mechanisms by which exercise training improves cardiac function and hypertrophy in the spontaneously hypertensive rats(SHR). Method:Male 5-wk old SHR and normotensive WKY rats were randomly divided into sedentary (S) and exercise training(E) groups, consisting of 4 groups as : SHR-S,SHR-E,WKY-S and WKY-E,n=10 for each group. The trained rats ran on a treadmill with low-to-moderate intensity for 8 weeks (60min/d,18—20m/s, 5d/wk). Systolic blood pressure (SBP) was measured by a tail-cuff method. The left ventricular end-diastolic pressure(LVEDP), the maximal increase and decrease velocity of left ventricular pressure (±dp/dt max)were recorded through the left ventricular cannula. The left ventricular mass index(LVMI) was assessed to represent cardiac hypertrophy. The mRNA expression of angiotensin converting enzyme(ACE) and angiotensinⅡ 1 type receptors (AT1) in the left ventricular myocardium were detected by real-time PCR and the protein level of ACE was determined by Western blot. The Cardiac AngⅡwas assayed by ELISA。 Result:Before exercise, there were no significant difference in systolic blood pressure between 5-wk SHR and age-matched WKY. At the end of exercise training, SBP,LVEDP and LVMI were significantly increased, but-dp/dt max was decreased in the SHR-S group compared with WKY-S rats(P<0.01). The mRNA expression of ACE and AT1, protein expression of ACE and AngⅡ level in the left ventricle myocardium were significantly increased in the SHR-S group compared with the WKY-S group (P<0.01). However, SBP, LVEDP and LVMI were significantly lower in the SHR-E group than those in the SHR-S group(P<0.01), but -dp/dt max was higher in the SHR-E group than that in the SHR-S group(P<0.05). The mRNA expression of ACE and AT1, ACE protein expression and Ang II level were reduced in the SHR-E group compared with those in the SHR-S group(P<0.01).There were slight changes in SBP, LVEDP, LVMI and ±dp/dt max in the WKY-E group compared with those in the WKY-S group, but those alteration did not reach statistical significance. No remarkable difference in the mRNA expression of ACE and AT1, the protein expression of ACE and AngⅡ level in the left ventricular myocardium were found between the WKY-E group and the WKY-S group. Conclusion:Exercise training in prehypertension postpones the progress of hypertension, prevents the reduction of diastolic function in the left ventricle and cardiac hypertrophy in SHR, which may be associated with the inhibition of ACE-AngⅡ-AT1 axis overactivity in the heart.
Keywords:prehypertension  exercise training  cardiac function  renin-angiotensin system
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