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王 强,施洪飞.红芪复方联合运动预处理对大鼠心肌缺血再灌注损伤保护作用[J].中国康复医学杂志,2017,(4):434~439
红芪复方联合运动预处理对大鼠心肌缺血再灌注损伤保护作用    点此下载全文
王 强  施洪飞
合肥师范学院体育科学学院,合肥,230601
基金项目:国家自然科学基金面上项目(81574044)
DOI:
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摘要:
      摘要 目的:探讨红芪复方联合运动预处理对大鼠心肌缺血再灌注损伤保护作用。 方法:健康雄性SD大鼠50只,随机分为假手术对照组(sham)、心肌缺血再灌注模型对照组(IR)、红芪复方预处理模型组(HP)、运动预处理模型组(EP)和运动联合红芪复方预处理模型组(EHP)。结扎左冠状动脉前降支复制大鼠心肌缺血再灌注模型,用放免法检测血清内皮素(ET)、6-酮前列腺素F1α(6-k-PGF1α)、血栓素B2(TXB2),用酶联免疫法(ELASA)检测血清心肌肌钙蛋白Ⅰ(cTnⅠ)、白介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)含量,用化学比色法检测心肌丙二醛(MDA)含量,用紫外分光光度法检测心肌超氧化物歧化酶(SOD)活性,用原子分光光度法检测心肌中钙离子(Ca2+)浓度。 结果:①与IR组相比,EP组、HP组和EHP组血清cTnⅠ漏出明显减少,有显著性差异,与EHP组相比,EP组和HP组中血清cTnⅠ值明显升高;②与IR组相比,EP组、HP组和EHP组心肌细胞胞浆中Ca2+浓度显著降低,与EHP组相比,EP组中心肌细胞胞浆中Ca2+浓度明显升高;③与IR组相比,EP组、HP组和EHP组血清ET明显降低,差异显著,HP组和EHP组血清TXB2降低,有显著性差异,HP组和EHP组6-k-PGF1α显著升高,有显著性差异;与EHP组相比,EP组ET、TXB2显著上升,EP组6-k-PGF1α明显降低;④与IR组相比,EP组、HP组和EHP组心肌中TNF-α和IL-6含量明显降低;与EHP组相比,HP组和EP组心肌中TNF-α和HP组IL-6含量显著上升;⑤与IR组相比,EP组、HP组和EHP组心肌中SOD活性升高,心肌中MDA下降,有显著差异;与EHP组相比,EP组心肌中SOD活性显著降低,心肌中MDA含量显著上升。 结论:红芪复方联合运动预处理可通过增强缺血再灌注心肌的抗氧化能力,减轻白细胞损伤,维持血管内皮功能稳定,改善微循环,降低钙超载,发挥对缺血再灌注心肌的保护效应。
关键词:心肌缺血再灌注  运动  红芪复方  预处理
Effects of Hongqi formular combined with exercise preconditioning against myocardial ischemic reperfusion injury in rats    Download Fulltext
College of Sport Science, Hefei Normal University, Hefei, 230601
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Abstract:
      Abstract Objective: To study the effects and mechanisms of Hongqi formular combined with exercise preconditioning against myocardial ischemic reperfusion injury in rats. Method: Fifty health male SD rats were randomly divided into sham, IR, EP, HP and EHP group. After establishing of EP animal model, the rats were ligated ramus descendens coronary artery to induce myocardial ischemic reperfusion model. The serum cardiac troponin Ⅰ (cTnⅠ), myocardium IL-6, TNF-α were detected by enzyme linked immunosorbent assay (ELISA). The myocardium MDA was detected by chemical colorimetry method. The myocardium SOD was detected by xanthine hydrocarbonylation. The serum ET, 6-k-PGF1α, TXB2 density were detected by radioimmunoassay, and the myocardium Ca2+ density was detected by atom absorption spectrophotometry. Result: ①As compared with the group IR, the serum cTnⅠ decreased significantly in groups EP, HP and EHP; As compared with the group EHP, the cTnⅠ level in serum was increased in groups EP and HP. ②As compared with the group IR, the Ca2+ level in myocardial sarcoplasmic reticulum was decreased significantly in groups EP, HP and EHP; As compared with the group EHP, the Ca2+ level in myocardial sarcoplasmic reticulum was decreased significantly in group EP. ③As compared with the group IR, the ET in serum were decreased significantly in groups EP, HP and EHP; As compared with the group EHP, the ET, TXB2 in serum were increased significantly in groups EP and the 6-k-PGF1αin serum were decreased significantly in groups EP. ④As compared with the group IR, the IL-6 and TNF-α in cardiac muscle were decreased in groups EP, HP and EHP; As compared with the group EHP, the IL-6 and TNF-α in cardiac muscle were increased in groups EP and HP. ⑤As compared with the group IR, the SOD in cardiac muscle was increased significantly in groups EP, HP and EHP and the MDA in cardiac muscle was decreased significantly in groups EP, HP and EHP; As compared with the group EHP, the SOD in cardiac muscle was decreased significantly in groups EP and the MDA in cardiac muscle was decreased significantly in group EP. Conclusion: Hongqi formular combined with exercise preconditioning produces cardio-protective effect during myocardial ischemic reperfusion, which may be related with the enhanced anti-oxidant ability, the decreased blood cell damage, the maintainace of vascular endothelium function, the improved microcirculation and the decreased calcium overloading.
Keywords:myocardial ischemic reperfusion  exercise  hongqi formula  preconditioning
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