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胡琰茹,刘晓莉,乔德才.“黑质/苍白球-丘脑-皮质”通路5-羟色胺1B受体及多巴胺1型受体在大鼠运动疲劳调控中的作用[J].中国康复医学杂志,2017,(5):501~508
“黑质/苍白球-丘脑-皮质”通路5-羟色胺1B受体及多巴胺1型受体在大鼠运动疲劳调控中的作用    点此下载全文
胡琰茹  刘晓莉  乔德才
北京师范大学体育与运动学院,北京,100875
基金项目:国家自然科学基金资助项目(31171138);湖南省体育科学学会重点资助项目(2015HN016)
DOI:
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摘要:
      摘要 目的:通过观察运动大鼠力竭前后“黑质/苍白球-丘脑-皮质”通路各核团5-羟色胺1B受体(5-HT1B)及多巴胺1型受体(D1DR)的蛋白表达水平,探讨“黑质/苍白球-丘脑-皮质”通路调控运动疲劳发生、发展的中枢机制。 方法:24只雄性Wistar大鼠,随机分为安静对照组、力竭即刻组、恢复90min组,每组8只。采用免疫组织化学技术对不同组别5-HT1B和D1DR的蛋白表达水平进行检测。 结果:与安静组对照组相比,力竭即刻组、恢复90min组大鼠黑质网状部及苍白球内侧部5-HT1B受体阳性细胞R值及平均光密度(AOD)值均显著减少(P<0.05),D1DR受体阳性细胞R值及AOD值均显著增加(P<0.05);力竭即刻组、恢复90min组大鼠丘脑腹外侧核5-HT1B受体阳性细胞R值及AOD值均显著增加(P<0.05,P<0.01);在大鼠皮质辅助运动区,力竭即刻组、恢复90min组5-HT1B受体阳性细胞R值及AOD值均显著增加(P<0.05),D1DR受体阳性细胞R值及AOD值均显著减少(P<0.05)。 结论:力竭运动过程中“黑质网状部/苍白球内侧部-丘脑-皮质”各核团5-HT1B及D1DR受体蛋白表达变化引起神经递质释放量改变,进而影响神经元电活动兴奋性,可能是“黑质网状部/苍白球内侧部-丘脑-皮质”通路调节运动疲劳及运动能力的重要途径之一。
关键词:大鼠  力竭运动  “黑质/苍白球-丘脑-皮质”通路  5-羟色胺1B受体  多巴胺1型受体
The modulating effect of 5-HT1B and D1DR receptors in neurons within “SNr/GPi-VL-SMA” pathway on exercise induced fatigue    Download Fulltext
College of Physical Education and Sports, Beijing Normal University, Beijing, 100875
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Abstract:
      Abstract Objective: To observe the changes of 5-HT1B and D1DR expression in nucleus of “SNr/GPi-VL-SMA” pathway in pre and post-exhaustive exercise, and investigate the central mechanism of “SNr/GPi-VL-SMA” pathway modulating the occurrence and development of exercise-induced fatigue. Method: Twenty-four male Wistar rats were randomly divided into control group (CG), immediately after exhaustion group (EG), and 90min after recovery group (RG), each consists of 8 rats. The expression level of 5-HT1B and D1DR receptors in each group were observed with the immunohistochemistry technique. Result: Compared with CG, the positive cell area/scanning area value (R value) and the average optical density value (AOD value) of 5-HT1B receptor in SNr and GPi of the EG and RG rats both decreased significantly (P<0.05), while the R value and AOD value of D1DR both increased significantly(P<0.05). The R value and AOD value of 5-HT1B receptor in VL of EG and RG rats increased significantly(P<0.05, P<0.01). In SMA, the R value and AOD value of 5-HT1B receptor of EG and RG rats increased significantly (P<0.05), while, the R value and AOD value of D1DR receptor decreased significantly (P<0.05). Conclusion: Changes of 5-HT1B receptor and D1DR receptor expression in the nuclei of ‘SNr/GPi-VL-SMA’ pathway caused the changes of the neurotransmitters release, thus affecting the excitability of the electrical activity of neurons, this might be one of the important ways for ‘SNr/GPi-VL-SMA’ pathway modulating the exercise-induced fatigue and exercise performance.
Keywords:rat  exhausting exercise  “SNr/GPi-VL-SMA” pathway  5-HT1B  D1DR
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