| 胡琰茹,刘晓莉,乔德才.“黑质/苍白球-丘脑-皮质”通路5-羟色胺1B受体及多巴胺1型受体在大鼠运动疲劳调控中的作用[J].中国康复医学杂志,2017,(5):501~508 |
| “黑质/苍白球-丘脑-皮质”通路5-羟色胺1B受体及多巴胺1型受体在大鼠运动疲劳调控中的作用 点此下载全文 |
| 胡琰茹 刘晓莉 乔德才 |
| 北京师范大学体育与运动学院，北京，100875 |
| 基金项目：国家自然科学基金资助项目（31171138）；湖南省体育科学学会重点资助项目（2015HN016） |
| DOI： |
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| 摘要： |
| 摘要
目的：通过观察运动大鼠力竭前后“黑质/苍白球-丘脑-皮质”通路各核团5-羟色胺1B受体（5-HT1B）及多巴胺1型受体（D1DR）的蛋白表达水平，探讨“黑质/苍白球-丘脑-皮质”通路调控运动疲劳发生、发展的中枢机制。
方法：24只雄性Wistar大鼠，随机分为安静对照组、力竭即刻组、恢复90min组，每组8只。采用免疫组织化学技术对不同组别5-HT1B和D1DR的蛋白表达水平进行检测。
结果：与安静组对照组相比，力竭即刻组、恢复90min组大鼠黑质网状部及苍白球内侧部5-HT1B受体阳性细胞R值及平均光密度（AOD）值均显著减少（P＜0.05），D1DR受体阳性细胞R值及AOD值均显著增加（P＜0.05）；力竭即刻组、恢复90min组大鼠丘脑腹外侧核5-HT1B受体阳性细胞R值及AOD值均显著增加（P＜0.05，P＜0.01）；在大鼠皮质辅助运动区，力竭即刻组、恢复90min组5-HT1B受体阳性细胞R值及AOD值均显著增加（P＜0.05），D1DR受体阳性细胞R值及AOD值均显著减少（P＜0.05）。
结论：力竭运动过程中“黑质网状部/苍白球内侧部-丘脑-皮质”各核团5-HT1B及D1DR受体蛋白表达变化引起神经递质释放量改变，进而影响神经元电活动兴奋性，可能是“黑质网状部/苍白球内侧部-丘脑-皮质”通路调节运动疲劳及运动能力的重要途径之一。 |
| 关键词：大鼠 力竭运动 “黑质/苍白球-丘脑-皮质”通路 5-羟色胺1B受体 多巴胺1型受体 |
| The modulating effect of 5-HT1B and D1DR receptors in neurons within “SNr/GPi-VL-SMA” pathway on exercise induced fatigue Download Fulltext |
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| College of Physical Education and Sports, Beijing Normal University, Beijing, 100875 |
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| Abstract: |
| Abstract
Objective: To observe the changes of 5-HT1B and D1DR expression in nucleus of “SNr/GPi-VL-SMA” pathway in pre and post-exhaustive exercise, and investigate the central mechanism of “SNr/GPi-VL-SMA” pathway modulating the occurrence and development of exercise-induced fatigue.
Method: Twenty-four male Wistar rats were randomly divided into control group (CG), immediately after exhaustion group (EG), and 90min after recovery group (RG), each consists of 8 rats. The expression level of 5-HT1B and D1DR receptors in each group were observed with the immunohistochemistry technique.
Result: Compared with CG, the positive cell area/scanning area value (R value) and the average optical density value (AOD value) of 5-HT1B receptor in SNr and GPi of the EG and RG rats both decreased significantly (P＜0.05), while the R value and AOD value of D1DR both increased significantly(P＜0.05). The R value and AOD value of 5-HT1B receptor in VL of EG and RG rats increased significantly(P＜0.05, P＜0.01). In SMA, the R value and AOD value of 5-HT1B receptor of EG and RG rats increased significantly (P＜0.05), while, the R value and AOD value of D1DR receptor decreased significantly (P＜0.05).
Conclusion: Changes of 5-HT1B receptor and D1DR receptor expression in the nuclei of ‘SNr/GPi-VL-SMA’ pathway caused the changes of the neurotransmitters release, thus affecting the excitability of the electrical activity of neurons, this might be one of the important ways for ‘SNr/GPi-VL-SMA’ pathway modulating the exercise-induced fatigue and exercise performance. |
| Keywords:rat exhausting exercise “SNr/GPi-VL-SMA” pathway 5-HT1B D1DR |
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