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董晓阳,刘 丹,黄菲菲,冯 珍.迷走神经电刺激对脑外伤后昏迷大鼠前额叶皮质和下丘脑Orexin-A及其受体OX1R表达变化的影响[J].中国康复医学杂志,2017,(7):744~749
迷走神经电刺激对脑外伤后昏迷大鼠前额叶皮质和下丘脑Orexin-A及其受体OX1R表达变化的影响    点此下载全文
董晓阳  刘 丹  黄菲菲  冯 珍
南昌大学第一附属医院康复医学科,南昌,330006
基金项目:国家自然科学基金项目(81260295);江西省研究生创新基金(YC2015-S090)
DOI:
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摘要:
      摘要 目的:探讨迷走神经电刺激(VNS)对脑外伤(TBI)昏迷大鼠的促醒作用及可能相关机制。 方法:将SD大鼠随机分为3组:空白对照组、假刺激组和刺激组。应用VNS治疗脑外伤后昏迷大鼠,观察其行为学变化,并用ELISA、Western-blot、免疫组织化学技术检测各组大鼠前额叶皮质(PFC)和下丘脑组织的Orexin-A及OX1R表达。 结果:刺激组30只大鼠中20只出现翻正反射,假刺激组30只中8只出现翻正反射;将3组的Orexin-A及OX1R含量进行两两比较,发现刺激组Orexin-A及受体OX1R水平高于假刺激组,差异有显著性意义(P<0.05)。 结论:VNS可提高脑外伤昏迷大鼠意识状态,其可能机制为上调PFC及下丘脑部位Orexin-A及OX1R水平,因此VNS有望成为脑外伤后昏迷促醒有效方法。
关键词:脑外伤  迷走神经电刺激  促醒  昏迷  Orexin-A  OX1R
Effects of vagus nerve stimulation on awaking up and upregulating expression of orexin-A and OX1R in prefrontal cortex and hypothalamic area in traumatic brain injury coma rats induced rats    Download Fulltext
Dept. of Rehabilitation Medicine, The First Affiliated Hospital of Nanchang University, Jiangxi, 330006
Fund Project:
Abstract:
      Abstract Objective: To explore the wake-promoting effects and possible mechanisms of vagus nerve stimulation (VNS) in traumatic brain injury induce-coma rats. Method: SD rats were randomly divided into three groups: control, sham-stimulated (TBI) and stimulated (TBI+VNS) group. ELISA, Western blot and immunohistochemistry technique were used to detect the expression of Orexin-A and OX1R level in prefrontal cortex (PFC) and hypothalamus area. Result: Twenty of 30 rats exhibited righting reflex in stimulated group, and 8 of 30 rats had the same responses in TBI groups. Orexin-A and OX1R levels in stimulated groups were higher than TBI groups both in PFC and hypothalamus area (P<0.05). Conclusion: VNS could raise consciousness in TBI induced coma rats, and the potential mechanism may be upregulating the expression of Orexin-A and OX1R in PFC and hypothalamus area. Therefore, VNS may be used as a wake-promoting method for patient after brain injury.
Keywords:vagus nerve stimulation  traumatic brain injury  wake-promoting  coma  Orexin-A  OX1R
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