| 董晓阳,刘 丹,黄菲菲,冯 珍.迷走神经电刺激对脑外伤后昏迷大鼠前额叶皮质和下丘脑Orexin-A及其受体OX1R表达变化的影响[J].中国康复医学杂志,2017,(7):744~749 |
| 迷走神经电刺激对脑外伤后昏迷大鼠前额叶皮质和下丘脑Orexin-A及其受体OX1R表达变化的影响 点此下载全文 |
| 董晓阳 刘 丹 黄菲菲 冯 珍 |
| 南昌大学第一附属医院康复医学科，南昌，330006 |
| 基金项目：国家自然科学基金项目（81260295）；江西省研究生创新基金（YC2015-S090） |
| DOI： |
| 摘要点击次数: 2011 |
| 全文下载次数: 1848 |
| 摘要： |
| 摘要
目的：探讨迷走神经电刺激（VNS）对脑外伤（TBI）昏迷大鼠的促醒作用及可能相关机制。
方法：将SD大鼠随机分为3组：空白对照组、假刺激组和刺激组。应用VNS治疗脑外伤后昏迷大鼠，观察其行为学变化，并用ELISA、Western-blot、免疫组织化学技术检测各组大鼠前额叶皮质（PFC）和下丘脑组织的Orexin-A及OX1R表达。
结果：刺激组30只大鼠中20只出现翻正反射，假刺激组30只中8只出现翻正反射；将3组的Orexin-A及OX1R含量进行两两比较，发现刺激组Orexin-A及受体OX1R水平高于假刺激组，差异有显著性意义（P＜0.05）。
结论：VNS可提高脑外伤昏迷大鼠意识状态，其可能机制为上调PFC及下丘脑部位Orexin-A及OX1R水平，因此VNS有望成为脑外伤后昏迷促醒有效方法。 |
| 关键词：脑外伤 迷走神经电刺激 促醒 昏迷 Orexin-A OX1R |
| Effects of vagus nerve stimulation on awaking up and upregulating expression of orexin-A and OX1R in prefrontal cortex and hypothalamic area in traumatic brain injury coma rats induced rats Download Fulltext |
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| Dept. of Rehabilitation Medicine, The First Affiliated Hospital of Nanchang University, Jiangxi, 330006 |
| Fund Project: |
| Abstract: |
| Abstract
Objective: To explore the wake-promoting effects and possible mechanisms of vagus nerve stimulation (VNS) in traumatic brain injury induce-coma rats.
Method: SD rats were randomly divided into three groups: control, sham-stimulated (TBI) and stimulated (TBI+VNS) group. ELISA, Western blot and immunohistochemistry technique were used to detect the expression of Orexin-A and OX1R level in prefrontal cortex (PFC) and hypothalamus area.
Result: Twenty of 30 rats exhibited righting reflex in stimulated group, and 8 of 30 rats had the same responses in TBI groups. Orexin-A and OX1R levels in stimulated groups were higher than TBI groups both in PFC and hypothalamus area (P＜0.05).
Conclusion: VNS could raise consciousness in TBI induced coma rats, and the potential mechanism may be upregulating the expression of Orexin-A and OX1R in PFC and hypothalamus area. Therefore, VNS may be used as a wake-promoting method for patient after brain injury. |
| Keywords:vagus nerve stimulation traumatic brain injury wake-promoting coma Orexin-A OX1R |
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