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张丽颖,胡昔权,郑海清,陈 曦,李莉莉,罗 婧.运动训练对脑梗死大鼠梗死边缘区神经细胞自噬及凋亡的影响[J].中国康复医学杂志,2017,(8):863~868
运动训练对脑梗死大鼠梗死边缘区神经细胞自噬及凋亡的影响    点此下载全文
张丽颖  胡昔权  郑海清  陈 曦  李莉莉  罗 婧
中山大学附属第三医院康复医学科,广州,510630
基金项目:国家自然科学基金面上项目(81372107),广东省自然科学基金重点项目(S2013020012648)
DOI:
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摘要:
      摘要 目的:研究运动训练对脑梗死急性期大鼠神经功能恢复及梗死边缘区皮质神经细胞自噬及凋亡的影响。 方法:建立大脑中动脉闭塞再灌注模型,并分成运动训练组、对照组和假手术组。运动训练组大鼠给予运动训练;对照组与假手术组则不予以任何针对性训练。造模术后第3、7、14d采用改良神经损伤程度评分(mNSS)对各组大鼠进行功能评估,同时以尼氏染色法测量脑梗死体积,免疫荧光方法观察梗死边缘区皮质自噬标志物LC3-Ⅱ及凋亡标志物TUNEL的表达情况。 结果:造模术后14d运动训练组大鼠mNSS及脑梗死体积均优于对照组,差别具有显著性意义(P<0.05)。运动训练组大鼠梗死边缘区皮质LC3-Ⅱ阳性细胞数在造模后14d少于对照组(P<0.05)。相关性分析结果显示LC3-Ⅱ阳性细胞数与mNSS(r=0.901,P<0.001)及梗死体积(r=0.832,P<0.001)成正相关。TUNEL阳性细胞数在造模后7、14d均少于对照组(P<0.001),且44.6%的LC3-Ⅱ与TUNEL存在共定位。 结论:运动训练能够促进脑梗死急性期大鼠神经功能恢复,其机制可能与其减少梗死边缘区神经细胞自噬及凋亡有关。
关键词:脑梗死  运动训练  自噬  凋亡  大鼠
Physical exercise mitigates autophagy and apoptosis after MCAO in rats    Download Fulltext
Department of Rehabilitation Medicine, the Third Affiliated Hospital, Sun Yat-sen University, Guangzhou,510630
Fund Project:
Abstract:
      Abstract Objective:To investigate the effects of exercise training on neurological function, autophagic cell death and apoptosis in cortex of peri-infarct region after transient left middle cerebral artery occlusion(MCAO) in rats. Method: A total of 30 male Sprague-Dawley rats were subjected to tMCAO and randomly divided into three groups: the rehabilitative training group (n=10), took running exercise everyday since 3 days after tMCAO;the control group (n=15) and sham-operated group (n=5, filament was not inserted into the artery), fed in standard cages with no special exercise training and served as controls. The animals were given behavioral tests with modified neurological severity scores(mNSS) to assess functional outcome. Nissl staining was performed to evaluate the infarct volume, and Immuno-fluorescence staining was employed to investigate autophagy, apoptosis in cortex of peri-infarct region at 3, 7, 14days after tMCAO, respectively. Result:The mNSS and infarct volume were much lower in rehabilitative training group than that in control group at 14 days after tMCAO (P<0.05). Immuno-fluorescence staining showed that autophagosomes, as labeled by microtubule-associated protein 1A light chain 3-II (LC3-Ⅱ), were evident in cortex of peri-infarct region at 3 days after tMCAO. The number of LC3-Ⅱ positive cells was significantly lower in rehabilitative training group than in control group at 14 day after tMCAO (P<0.05). In addition, physical exercise significantly decreased the number of TUNEL positive cells at 7, 14days after tMCAO(P<0.001). Moreover, 44.6% of LC3-Ⅱ positive cells were also stained with TUNEL. Conclusion: The present results demonstrate that physical exercise enhances neurological function possibly by reduction of autophagosome accumulation, and apoptosis in cortex of peri-infarct region after tMCAO in rats.
Keywords:cerebral infarction  physical exercise  autophagy  apoptosis  rat
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