| 项 洁,吴 鑫,陆 晓,励建安.心肌远隔缺血预适应通过SDF-1α/CXCR4途径保护兔心肌缺血/再灌注损伤的研究[J].中国康复医学杂志,2017,(10):1101~1107 |
| 心肌远隔缺血预适应通过SDF-1α/CXCR4途径保护兔心肌缺血/再灌注损伤的研究 点此下载全文 |
| 项 洁 吴 鑫 陆 晓 励建安 |
| 徐州医科大学附属医院康复医学科，江苏徐州，221000 |
| 基金项目：国家自然科学基金面上项目(81472164)；徐州市社会发展项目（XM13B060） |
| DOI： |
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| 摘要
目的：探讨远隔缺血预适应对心肌缺血/再灌注(I/R)损伤保护作用及潜在机制。
方法：成年雄性新西兰大白兔随机分成：①心肌缺血再灌注组（I/R组，n=6）；②假手术组（Sham，n=6）；③远隔缺血预适应（RIPC+I/R组，n=6)；④SDF-1α/CXCR4阻断剂AMD3100+I/R组，（n=6）；⑤AMD3100+RIPC+I/R组，（n=6）。ELISA检测外周血中SDF-1α；TTC检测心肌梗死面积；TUNEL检测细胞凋亡率；Western Blot检测Bcl-2、Bax蛋白表达。
结果：与Sham组相比，I/R组及RIPC+I/R组都可促进外周血中SDF-1α的释放，且后者的作用更显著(P<0.05）；RIPC+I/R及AMD3100+RIPC+I/R组可以不同程度缩小心肌梗死面积及降低心肌细胞凋亡率(P<0.05），AMD3100+RIPC+I/R组与RIPC+I/R组相比，心肌梗死面积及细胞凋亡率均有所增加(P<0.05）。
结论：远隔缺血预适应可能通过SDF-1α/CXCR4信号通路对心缺血再灌注损伤心肌发挥保护作用。 |
| 关键词：远隔缺血预适应 心肌缺血/再灌注损伤 基质细胞衍生因子 |
| The cardioprotection effects of remote ischemic preconditioning on myocardial ischemia reperfusion injury through SDF-1α/CXCR4 signal pathway Download Fulltext |
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| The Affiliated Hospital of Xuzhou Medical University, Xuzhou, Jiangsu, 221000 |
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| Abstract: |
| Abstract
Objective: To investigate the protective effect of remote ischemic preconditioning(PIPC) on myocardial ischemia/reperfusion(I/R) injury and underlying mechanism.
Method: Clean level male white rabbits of New Zealand, weight 2.2—2.5 kg, were randomly divided into the following groups: Sham (n=6), myocardial I/R(n=6), RIPC+I/R (n=6), SDF-1α/CXCR4 inhibitor(AMD3100)+I/R, AMD3100+RIPC+I/R. The release of SDF-1α was detected by ELISA from the serum, the size of myocardial infarction was assayed by TTC, cardiomyocyte apoptosis rate was detected by TUNEL, and the expressions of Bcl-2，Bax were assessed via western blot.
Result: Myocardial I/R and RIPC+I/R can promote the expressions of SDF-1α compared with Sham group(P<0.05）, more obvious in RIPC+I/R group(P<0.05); Compared with I/R group, myocardium infarction area and cardiomyocyte apoptosis rate were reduced between RIPC+I/R and AMD3100+RIPC+I/R, with the increase of Bcl-2，the decrease of Bax(P<0.05）. By comparing with RIPC+I/R group, myocardium infarction area and cardiomyocyte apoptosis rate were increased in AMD3100+RIPC+I/R group(P<0.05）.
Conclusion: RIPC can promote SDF-1α release to exert the cardioprotection effect against myocardium ischemia reperfusion injury via SDF-1α/CXCR4 signal pathway. |
| Keywords:remote ischemic preconditioning ischemia/reperfusion stromal cell derived factor-1 |
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