设为首页
加入收藏
联系我们
Email-Alert
 

    首页 | 杂志介绍 | 编委成员 | 审稿专家名录 | 投稿指南 | 订阅指南 | 过刊浏览 | 论著模板 | 综述模板 | 帮助

 
黄菲菲,董晓阳,陈 琴,冯 珍.迷走神经电刺激对脑外伤昏迷大鼠前额叶皮质组胺H1受体表达的影响[J].中国康复医学杂志,2017,(11):1208~1213
迷走神经电刺激对脑外伤昏迷大鼠前额叶皮质组胺H1受体表达的影响    点此下载全文
黄菲菲  董晓阳  陈 琴  冯 珍
南昌大学第一附属医院康复医学科,南昌,330006
基金项目:国家自然科学基金资助项目(81260295)
DOI:
摘要点击次数: 485
全文下载次数: 359
摘要:
      摘要 目的:探讨迷走神经电刺激(vagus nerve electrical stimulation, VNS)对脑外伤(traumatic brain injury,TBI)昏迷大鼠的促醒作用及其对TBI昏迷大鼠前额叶皮层组胺H1受体表达的影响。 方法:将72只SD大鼠分为4组:空白对照组、假刺激组(TBI)、刺激组(TBI+VNS)和Orexin-1受体(OX1R)拮抗剂组(TBI+SB334867+VNS)。应用VNS治疗脑外伤后昏迷大鼠,观察其行为学变化,并用免疫组织化学检测各组大鼠前额叶皮质组胺H1受体(H1R)表达的变化。 结果:刺激组18只大鼠中12只出现翻正反射;假刺激组4只出现翻正反射;拮抗剂组9只出现翻正反射。免疫组化结果显示刺激组大鼠PFC区的H1R的表达水平显著高于假刺激组和拮抗剂组(P<0.05)。 结论:VNS可改善脑外伤后昏迷大鼠的意识状态,有望成为TBI昏迷促醒的一种新的电刺激方法,其促醒机制可能与PFC区H1R水平的上调有关,Orexin-A可能通过OX1R在其中起调节作用。
关键词:迷走神经电刺激  脑外伤  昏迷  促醒  组胺H1受体  食欲素A
Expression of the histamine H1 receptor in rat prefrontal cortex with traumatic brain injury induced-coma after vagus nerve electrical stimulation    Download Fulltext
Department of Rehabilitation Medicine, the First Affiliated Hospital of Nanchang University, Medical School of Nanchang University, Nanchang, 330006
Fund Project:
Abstract:
      Abstract Objective: To investigate the wake-promoting action and the expression of histamine H1 receptor in rat prefrontal cortex (PFC) in traumatic brain injury (TBI)-coma rats after vagus nerve electrical stimulation (VNS). Method: Seventy-two SD rats were randomly divided into four groups: a control group, a sham stimulated group (TBI), a stimulated group (TBI+VNS), and an antagonist group (TBI+SB334867+VNS). VNS was applied to the rats after TBI-coma models were established and then a six classical levels of consciousness were assessed, followed by immunohistochemistry to determined the expression of the H1R in rat PFC at 6, 12, and 24 hours after injury. Result: The righting reflex was appeared in 12 rats in stimulated group, 4 rats in TBI groups, and 9 rats in antagonist group. Significant increase of H1R expression was observed in stimulated group when compared with sham stimulated group and antagonist group (P<0.05). Conclusion: The vagus nerve electrical stimulation might modulate wakefulness by promoting the the activity of H1R expression in the prefrontal cortex of rats with traumatic brain injury-induced coma, and Orexin-A and its receptor, OX1R may be involved in this modulation.
Keywords:vagus nerve electrical stimulation  traumatic brain injury  wake-promoting  coma  histamine H1 receptor  Orexin-A
查看全文  查看/发表评论  下载PDF阅读器

您是本站第 15396618 位访问者

版权所有:中国康复医学会
主管单位:国家卫生和计划生育委员会 主办单位:中国康复医学会
地址:北京市朝阳区樱花园东街,中日友好医院内   邮政编码:100029   电话:010-64218095   

本系统由北京勤云科技发展有限公司设计 京ICP备10000329号