| 黄菲菲,董晓阳,陈 琴,冯 珍.迷走神经电刺激对脑外伤昏迷大鼠前额叶皮质组胺H1受体表达的影响[J].中国康复医学杂志,2017,(11):1208~1213 |
| 迷走神经电刺激对脑外伤昏迷大鼠前额叶皮质组胺H1受体表达的影响 点此下载全文 |
| 黄菲菲 董晓阳 陈 琴 冯 珍 |
| 南昌大学第一附属医院康复医学科，南昌，330006 |
| 基金项目：国家自然科学基金资助项目（81260295） |
| DOI： |
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| 摘要
目的：探讨迷走神经电刺激(vagus nerve electrical stimulation, VNS)对脑外伤(traumatic brain injury,TBI)昏迷大鼠的促醒作用及其对TBI昏迷大鼠前额叶皮层组胺H1受体表达的影响。
方法：将72只SD大鼠分为4组：空白对照组、假刺激组（TBI）、刺激组(TBI+VNS)和Orexin-1受体(OX1R)拮抗剂组(TBI+SB334867+VNS)。应用VNS治疗脑外伤后昏迷大鼠，观察其行为学变化，并用免疫组织化学检测各组大鼠前额叶皮质组胺H1受体（H1R）表达的变化。
结果：刺激组18只大鼠中12只出现翻正反射；假刺激组4只出现翻正反射；拮抗剂组9只出现翻正反射。免疫组化结果显示刺激组大鼠PFC区的H1R的表达水平显著高于假刺激组和拮抗剂组（P＜0.05）。
结论：VNS可改善脑外伤后昏迷大鼠的意识状态，有望成为TBI昏迷促醒的一种新的电刺激方法，其促醒机制可能与PFC区H1R水平的上调有关，Orexin-A可能通过OX1R在其中起调节作用。 |
| 关键词：迷走神经电刺激 脑外伤 昏迷 促醒 组胺H1受体 食欲素A |
| Expression of the histamine H1 receptor in rat prefrontal cortex with traumatic brain injury induced-coma after vagus nerve electrical stimulation Download Fulltext |
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| Department of Rehabilitation Medicine, the First Affiliated Hospital of Nanchang University, Medical School of Nanchang University, Nanchang, 330006 |
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| Abstract: |
| Abstract
Objective: To investigate the wake-promoting action and the expression of histamine H1 receptor in rat prefrontal cortex (PFC) in traumatic brain injury (TBI)-coma rats after vagus nerve electrical stimulation (VNS).
Method: Seventy-two SD rats were randomly divided into four groups: a control group, a sham stimulated group (TBI), a stimulated group (TBI+VNS), and an antagonist group (TBI+SB334867+VNS). VNS was applied to the rats after TBI-coma models were established and then a six classical levels of consciousness were assessed, followed by immunohistochemistry to determined the expression of the H1R in rat PFC at 6, 12, and 24 hours after injury.
Result: The righting reflex was appeared in 12 rats in stimulated group, 4 rats in TBI groups, and 9 rats in antagonist group. Significant increase of H1R expression was observed in stimulated group when compared with sham stimulated group and antagonist group (P＜0.05).
Conclusion: The vagus nerve electrical stimulation might modulate wakefulness by promoting the the activity of H1R expression in the prefrontal cortex of rats with traumatic brain injury-induced coma, and Orexin-A and its receptor, OX1R may be involved in this modulation. |
| Keywords:vagus nerve electrical stimulation traumatic brain injury wake-promoting coma histamine H1 receptor Orexin-A |
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