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廖诚诚,孙伟铭,冯 珍.经颅直流电刺激对脑外伤昏迷大鼠意识及脑源性生长因子表达的影响[J].中国康复医学杂志,2018,(3):269~273
经颅直流电刺激对脑外伤昏迷大鼠意识及脑源性生长因子表达的影响    点此下载全文
廖诚诚  孙伟铭  冯 珍
南昌大学第一附属医院,南昌,330006
基金项目:江西省教育厅科学技术研究项目
DOI:
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摘要:
      摘要 目的:探讨经颅直流电刺激(tDCS)对脑外伤昏迷大鼠的促醒作用及其可能机制。 方法:成年SD大鼠54只,随机分为3组,每组18只,分别是空白组、脑外伤(TBI)昏迷组及tDCS组。TBI和tDCS组制作脑外伤昏迷模型,前者进行假tDCS治疗,后者用tDCS治疗。各组处理后在6h、12h、24h点评定大鼠意识水平,对比治疗前后意识水平变化,处死大鼠,获取前额叶和海马,应用Western Blot法测定大鼠组织中脑源性生长因子(BDNF)的表达。 结果:空白组18只、TBI组6只、tDCS组11只出现翻正反射,TBI组大鼠前额叶与海马BDNF表达较空白组升高,在12h点,tDCS组大鼠前额叶BDNF表达较TBI组升高,差异有显著性意义(P<0.05),在6h点,tDCS组大鼠海马BDNF表达较TBI组升高,差异有显著性意义(P<0.05)。 结论:tDCS可促TBI昏迷大鼠觉醒,其机制可能与上调前额叶及海马BDNF表达有关。
关键词:经颅直流电刺激  脑外伤  昏迷  促醒  脑源性生长因子
Effects of transcranial direct current stimulation on consciousness and expression of brain-derived neurotrophic factor in coma rats following traumatic brain injury    Download Fulltext
Department of Rehabilitation Medicine, the First Affiliated Hospital of Nanchang University, Nanchang, 330006
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Abstract:
      Abstract Objective: To investigate wake-promoting effects of transcranial direct current stimulation (tDCS) on brain injury-induced coma and the possible mechanism. Method: Fifty-four adult SD rats were randomly divided into three groups with 18 rats in each group.They were blank group, traumatic brain injury-induced coma (TBI) group and tDCS group. Using classical free fall method to create brain injury-induced coma and then treated rats with tDCS, consciousness level of rats were assessed at 6h, 12h, 24h time points. After consciousness level evaluation, rats were put to death and then the prefrontal cortex (PFC) and hippocampus of rats were extracted. Western Blot method was used to determine the expression of brain-derived neurotrophic factor (BDNF) in three groups. Result: Eighteen rats in control group, 6 rats in TBI group and 11 rats in tDCS group awakened. BDNF expression in TBI group was higher than that in blank group in PFC and hippocampus. More over, at 12h in PFC and at 6h in hippocampus, BDNF expression in tDCS group was higher than that in TBI group with statistically significant difference(P<0.05). Conclusion: tDCS can improve the consciousness level of coma rats following TBI and the mechanism may be related to upregulation of BDNF expression in the PFC and hippocampus of rats.
Keywords:transcranial direct current stimulation  traumatic brain injury  coma  wake-promoting  brain-derived neurotrophic factor
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