| 徐春燕,陈禹彤,欧吉兵,肖熠斐,刘 锋,姚黎清.冲击波对兔复合肌肉动作电位及肌组织形态学的影响[J].中国康复医学杂志,2018,(9):1019~1023 |
| 冲击波对兔复合肌肉动作电位及肌组织形态学的影响 点此下载全文 |
| 徐春燕 陈禹彤 欧吉兵 肖熠斐 刘 锋 姚黎清 |
| 昆明医科大学第二附属医院,昆明,650000 |
| 基金项目：云南省教育厅科学研究基金项目（2015C022Y） |
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| 摘要
目的：评估单侧小腿后方放散式体外冲击波（radial extracorporeal shock wave treatment,rESWT）处理后兔的双侧腓肠肌复合肌肉动作电位和肌组织形态学。
方法：体重2±0.2kg雄性新西兰兔63只，其中3只兔进行预实验确定rESWT强度，对剩余60只兔左小腿三头肌肌腹最粗大的位置稍偏外侧行强度1.5bar，频率10Hz的2000次冲击。分为6组，在放散式体外冲击波处理当天和处理后第1、2、4、6、8周在麻醉情况下用日本光电MEB-9100K肌电图仪记录双侧腓肠肌外侧头复合肌肉动作电位，比较两侧波幅、潜伏期，每组抽取一个样本对其双侧腓肠肌外侧头行重复电刺激，记录不同频率刺激后波幅衰减情况。肌电图检查完成立即取下双侧腓肠肌外侧头肌组织，冰冻切片后进行HE染色，观察肌肉组织形态学变化。
结果：前三组实验兔（处理当天和处理后第1、2周）实验侧动作电位波幅降低，与对照侧比较差异显著（P＜0.05），后三组实验兔实验侧动作电位波幅与对照侧无明显差异。所有实验兔双侧动作电位潜伏期差异不明显，HE染色未见肌组织形态学明显异常。
结论：运用rESWT对兔小腿三头肌肌腹最肥大处行强度1.5bar，频率10Hz的2000次冲击可降低其复合肌肉动作电位的波幅但作用持续时间较短且对肌组织形态学不会产生较大影响。 |
| 关键词：放散式体外冲击波 复合肌肉动作电位 HE染色 |
| Effects of shock wave on compound muscle action potential and muscle morphology in rabbits Download Fulltext |
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| The Second Affiliated Hospital of Kunming Medical University, Kunming,650000 |
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| Abstract: |
| Abstract
Objective:Evaluate the compound muscle action potential and the morphology of the muscle in the bilateral gastrocnemius after unilateral radial extracorporeal shock wave therapy.
Method：Totally 63 male New Zealand rabbits with weight in 2±0.2kg were used in this study. Three rabbits were pretested to determine the radial extracorporeal shock wave intensity. Two thousand shock waves at an energy flux density of 1.5bar and the frequency of 10Hz were applied to the slightly outside of the most bulky position in the left calf muscles.Divided into six groups,the compound muscle action potential of bilateral triceps surae was recorded by Japanese photoelectric MEB-9100K electromyogram instrument on the day of radial extracorporeal shock wave treatment and 1,2,4,6,8 weeks after radial extracorporeal shock wave treatment. The amplitudes and latencies were compared. One rabbit in each group was extracted by repeated electrical stimulation of its bilateral triceps surae. The amplitude decline after different frequency stimulation was recorded. Immediately after EMG was completed, HE staining was performed and the morphological changes of muscle were observed.
Result：The action potential amplitude of experimental side decreased in the first three groups(on the day of treatment and 1,2 weeks after treatment ) and the difference was significant compared with the control side(P＜0.05). There had no significant difference when compared with the control side for the latter three groups. The difference was not obvious about the action potential latency of bilateral triceps surae in all experimental rabbits. HE staining showed no obvious abnormality of muscle morphology.
Conclusion：Suitable dose of rESWT can not only reduce the amplitude of compound muscle action potential although the effect lasted for a short time, but also have no a greater impact on morphology of muscle tissue. |
| Keywords:radial extracorporeal shock wave treatment compound muscle action potential HE staining |
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