| 焦佳伟,王 鹏,张留超,何雄文,牛美兰,李萌萌,李 玲,张振香.常压氧对大鼠脑缺血再灌注损伤Na+-K+-2Cl-共转运蛋白1型的影响[J].中国康复医学杂志,2020,(8):932~937 |
| 常压氧对大鼠脑缺血再灌注损伤Na+-K+-2Cl-共转运蛋白1型的影响 点此下载全文 |
| 焦佳伟 王 鹏 张留超 何雄文 牛美兰 李萌萌 李 玲 张振香 |
| 郑州大学护理与健康学院基础教研室，河南省郑州市，450001 |
| 基金项目：河南省科技厅项目（162102310234，192102310351）；河南省教育厅创新人才项目（20HASTIT047） |
| DOI：10.3969/j.issn.1001-1242.2020.08.009 |
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| 摘要： |
| 摘要
目的：研究常压氧（NBO）对大鼠脑缺血再灌注损伤Na+-K+-2Cl-共转运蛋白1型（NKCC1）的影响。
方法：采用线栓法构建大鼠大脑中动脉缺血模型（MCAO），术后2h进行神经功能评分，将其评分与假手术组进行比较。MCAO模型构建成功者进行再灌注，随机分为NBO组和模型组，NBO组于再灌注后采用体积分数60%NBO进行治疗。各组大鼠分别在0、2、4、6、12和24h处死，取脑组织进行HE染色及Western Blot检测。
结果：与假手术组相比，MCAO组神经功能评分显著增高，差异有显著性意义（P<0.05）。HE染色结果显示模型组和NBO组大鼠缺血2h时脑组织均无明显病理结构改变，随着再灌注时间的延长，模型组大鼠脑组织病理改变逐渐增加，而与模型组相比，NBO组大鼠的脑组织病理改变明显减轻。Western Blot检测结果显示模型组和NBO组在起始2h内，脑组织NKCC1蛋白和缺氧诱导因子-1α（HIF-1α）蛋白表达水平与正常脑组织相比没有明显变化（P>0.05），随着再灌注时间的延长，模型组NKCC1蛋白和HIF-1α蛋白表达水平明显增加，NBO组NKCC1蛋白和HIF-1α蛋白表达水平虽有增加但其表达水平仍明显低于模型组（P<0.05）。
结论：NBO疗法可以下调脑缺血再灌注后脑组织NKCC1蛋白和HIF-1α蛋白的表达，减轻大鼠脑缺血再灌注损伤，促进大鼠神经功能恢复，具有脑损伤保护作用。 |
| 关键词：常压氧 Na+-K+-2Cl-共转运蛋白1型 缺血性脑卒中 |
| Effects of normal baric oxygen on Na+-K+-Cl- cotransporter1 in rats with cerebral ischemia-reperfusion injury Download Fulltext |
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| Nursing and Health School of Zhengzhou University, Zhengzhou, 450001 |
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| Abstract: |
| Abstract
Objective:To study the effects of normal baric oxygen (NBO) on Na+-K+-2Cl-cotransporter1 (NKCC1) in rats with cerebral ischemia-reperfusion injury.
Method:A rat model of middle cerebral artery occlusion (MCAO) was established by thread embolization. The neurological function was scored 2 hours after operation and compared with the sham operation group. Successful MCAO models were randomly divided into NBO group and model group. The NBO group was treated with 60% normobaric oxygen after reperfusion. Rats in each group were sacrificed at 0, 2, 4, 6,12 and 24h respectively. Brain tissues were taken for HE staining and Western blot.
Result:Compared with sham operation group, the neurological function score of MCAO group was significantly higher. HE staining showed that there were no obvious pathological changes in brain tissue in model group and NBO group after 2 hours of ischemia. The pathological changes of brain tissue of model group increased gradually with the prolongation of reperfusion. Compared with the model group, the pathological changes of brain tissue in NBO group were significantly alleviated. Western Blot results showed that the expression levels of NKCC1 protein and hypoxia inducible factor-1α (HIF-1α) protein in brain tissues of model group and NBO group did not change significantly compared with normal brain tissues within 2 hours after the onset of reperfusion(P>0.05). With the prolongation of reperfusion time, the expression levels of NKCC1 protein in model group increased significantly(P>0.05), and the expression levels of NKCC1 protein and HIF-1α protein in NBO group increased lesser than those in model group(P<0.05).
Conclusion:NBO therapy can down-regulate the expression of NKCC1 and HIF-1α in brain tissue after cerebral ischemia-reperfusion, alleviate cerebral ischemia-reperfusion injury in rats, promote the recovery of neurological function, and has protective effect on brain injury. |
| Keywords:normobaric oxygen Na+-K+-2Cl- cotransporter 1 ischemic stroke |
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