| 王 渊,王 强,樊恩召,任 博,郭 婷,刘智斌,刘国强.嗅三针对SAMP8小鼠海马磷酸化PI3K/Akt蛋白表达和突触可塑性的影响[J].中国康复医学杂志,2021,(2):135~142 |
| 嗅三针对SAMP8小鼠海马磷酸化PI3K/Akt蛋白表达和突触可塑性的影响 点此下载全文 |
| 王 渊 王 强 樊恩召 任 博 郭 婷 刘智斌 刘国强 |
陕西中医药大学针灸推拿学院，陕西省针药结合重点实验室/咸阳市神经生物学(针灸)
重点实验室，咸阳，712046 |
| 基金项目：国家自然科学基金项目（82074552，81503667）；陕西省科技厅自然科学基础研究计划项目（2018JM7041）；陕西中医药大学经脉-脏腑相关研究创新团队项目（2019-YL09） |
| DOI：10.3969/j.issn.1001-1242.2021.02.002 |
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| 摘要： |
| 摘要
目的：观察嗅三针对SAMP8小鼠海马磷酸化PI3K、Akt蛋白表达和海马突触可塑性损伤的影响，探讨嗅三针治疗阿尔茨海默病（AD）模型小鼠的作用机制。
方法：将40只SAMP8小鼠随机分为模型组（Model）、嗅三针组（XSZ）、嗅神经切断嗅三针组（XSZ+XSJ）和盐酸多奈哌齐组（Donepezil），每组10只，10只SAMR1小鼠作为正常组（Control）。XSZ组和XSZ+XSJ组采用电针刺激印堂穴和双侧迎香穴，Donepezil组采用盐酸多奈哌齐灌胃，Control组和Model组不干预。采用Morris水迷宫实验评价小鼠学习记忆能力，新物体识别实验评价小鼠新物体识别能力，Western Blot和免疫组化检测小鼠海马区p-PI3K和p-Akt蛋白表达，透射电镜观测小鼠海马突触超微结构。
结果：与Model组比较，XSZ和Donepezil干预均可以明显降低SAMP8小鼠4d平均逃避潜伏期，增加其靶象限停留时间、穿越平台次数和新物体偏爱指数，明显升高其海马p-PI3K和p-Akt蛋白表达（P<0.05），改善其海马神经元突触结构；XSZ+XSJ对于SAMP8小鼠学习记忆能力、新物体识别能力指标及p-PI3K、p-Akt蛋白表达无显著影响（P>0.05），对其突触结构损伤无明显改善；对于上述指标的影响，XSZ组与Donepezil组比较无显著差异（P>0.05）。
结论：嗅三针可能基于嗅觉通路完整性，调节海马PI3K/Akt磷酸化表达，修复海马突触形态结构损伤，改善海马突触可塑性，从而发挥提高SAMP8小鼠学习记忆能力的效应。 |
| 关键词：嗅三针 阿尔茨海默病 p-PI3K p-Akt 突触可塑性 |
| Effects of olfactory three needle on expression of phosphorylated PI3K/Akt protein and synaptic plasticity in the hippocampus of SAMP8 mice Download Fulltext |
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| College of Acupuncture Moxibustion and Tuina，Shaanxi University of Chinese Medicine，Xianyang，712046 |
| Fund Project: |
| Abstract: |
| Abstract
Objective：To explore mechanism of olfactory three needle therapy on AD model mice by observing its effects on expression of phosphorylated PI3K and Akt protein and damage of synaptic plasticity in the hippocampus of SAMP8 mice.
Method：Forty SAMP8 mice were randomly divided into the model group， the olfactory three needle group（XSZ），the olfactory three needle group with olfactory nerve transected（XSZ+XSJ）and the donepezil HCL group（Donepezil），with 10 mice in each group，and 10 SAMR1 mice as the control group. The XSZ group and the XSZ+XSJ group were stimulated by electroacupuncture at Yintang（EX-HN3）and bilateral Yingxiang（LI20）points，the Donepezil group was given intragastric administration of donepezil HCL，no intervention were given to the control group and the model group. Morris water maze test was used to evaluate the learning and memory ability, new object recognition experiment was used to evaluate the new object recognition ability. Western blot and immunohistochemistry were used to detect the expression of p-PI3K and p-Akt proteins in hippocampus of mice，and transmission electron microscopy was used to observe synaptic ultrastructure in hippocampus of mice.
Result： Compared with the model group，XSZ and Donepezil intervention could significantly reduce the 4-day average escape latency，increase the target quadrant residence time，platform crossing times and new object preference index，increase the expression of p-PI3K and p-Akt in the hippocampus of SAMP8 mice(P<0.05)，and improve the hippocampus synaptic structure of SAMP8 mice；the XSZ+XSJ group had no significant changes on learning and memory ability，new object recognition ability and p-PI3K，p-Akt protein expression (P>0.05)， and had no significant improvement on the damage to synaptic structure of SAMP8 mice. There was no significant difference between the XSZ group and the Donepezil group for the above indexes(P>0.05).
Conclusion： Olfactory three needle might regulate the expression of phosphorylated PI3K/Akt， repair the damage of synaptic morphology and structure，and improve the plasticity of synapses based on the integrity of olfactory pathway，thus exerting the effect of improving learning and memory ability of SAMP8 mice. |
| Keywords:olfactory three needle Alzheimer’s disease p-PI3K p-Akt synaptic plasticity |
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