| 褚昕宇,王泽军.跑转轮运动与限制性应激对小鼠恐惧记忆与焦虑样行为的影响[J].中国康复医学杂志,2021,(4):406~411 |
| 跑转轮运动与限制性应激对小鼠恐惧记忆与焦虑样行为的影响 点此下载全文 |
| 褚昕宇 王泽军 |
| 上海工程技术大学体育教学部，上海，201620 |
| 基金项目：国家社会科学基金青年项目（17CTY019） |
| DOI：10.3969/j.issn.1001-1242.2021.04.004 |
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| 摘要： |
| 摘要
目的：探讨自愿跑转轮运动与长期限制性应激对小鼠恐惧记忆与焦虑样行为的影响与海马脑源性神经营养因子的关系。
方法：将10周龄雄性C57Bl/6J小鼠80只随机分为8组，每组10只。其中，对照组(C1、C2)小鼠不接受运动或应激等任何行为干预方式的刺激，运动组(R1、R2)小鼠笼内安装有跑转轮装置，应激组(S1、S2)小鼠接受长期限制性应激，应激-运动组(S-R1、S-R2)小鼠笼内安装有跑转轮装置，并且接受长期限制性应激。另外，用于行为测试的(C2、S2、R2、S-R2)小鼠依次进行条件恐惧测试和高架十字迷宫测试，用于生化实验的(C1、S1、R1、S-R1)小鼠使用免疫印记法测定海马BDNF蛋白含量。
结果：在情境条件恐惧测试，应激组与应激-运动组小鼠较对照组的冰点行为时间百分比存在着显著性(P<0.05)减少；但在线索条件恐惧测试中，各组小鼠的冰点行为时间百分比没有明显差异。应激-运动组小鼠较运动组在闭合臂以及开放臂的停留时间存在显著性(P<0.05)差异，应激-运动组小鼠较运动组进入开放臂的次数出现显著性(P<0.05)增加。各组小鼠海马BDNF蛋白含量之间没有明显差异，只有应激组小鼠较对照组表现出明显升高的趋势(P=0.070)。
结论：应激损害了成年小鼠的情境恐惧记忆能力，但提高了海马BDNF蛋白水平。在条件恐惧测试后进行的高架十字迷宫实验中发现，应激-运动组小鼠的焦虑样行为明显少于运动组，认为先于条件恐惧测试进行的长期限制性应激似乎有助于维持6周跑转轮运动的抗焦虑作用。 |
| 关键词：运动 应激 焦虑 恐惧 脑源性神经营养因子 |
| Influences of wheel running and restrict stress on fear memory and anxiety-like behavior in mice Download Fulltext |
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| Shanghai University of Engineering Science,Shanghai,201620 |
| Fund Project: |
| Abstract: |
| Abstract
Objective： To investigate the influence of voluntary running wheel and chronic restrict stress on fear memory and anxiety-like behavior in mice and its relationship with brain-derived neurotrophic factor in the hippocampus.
Method： Ten weeks-old male C57Bl/6J mice were randomly divided into eight groups， 10 mice in each group. C1 and C2 mice were not given any interventions such as stress or exercise， R1 and R2 mice were housed with running wheel equipment， S1 and S2 mice received chronic restraint stress， S-R1 and S-R2 mice were housed with running wheel equipment，as well as received chronic restraint stress. The mice received behavioral tests(C2， S2， R2， S-R2) including the fear conditioning, and next the elevated-plus maze test. Western Blot was also given to the mice(C1， S1， R1， S-R1) for quantitative analysis of the level of hippocampal BDNF protein.
Result： In the contextual fear conditioning， the percentage of freezing time of stress mice and the stress-exercise mice was significantly reduced (P<0.05) compared with the control. However， there was no difference among each group about the percentage of freezing time in the cued fear conditioning test. There was significant difference (P<0.05) between the stress-exercise mice and the running mice in the time in closed arm and open arm during the elevated-plus maze test， and the stress-exercise mice exhibited significantly increased entries (P<0.05) to the open arm than the exercise mice. Although there is no difference in hippocampal BDNF protein levels among each group，the stress mice showed strong trends toward an increase (P=0.070).
Conclusion： Stress impairs contextual memory in adult mice， and increase hippocampal BDNF protein. In the elevated-plus maze test after fear conditioning， the stress-exercise mice showed significantly lower level of anxiety-like behavior than the running mice，suggesting that chronic restraint stress sustained the anxiolytic effect of exercise from footshock during fear conditioning training. |
| Keywords:exercise stress anxiety fear brain-derived neurotrophic factor |
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