| 赵晓红,严会荣,任 钰,牛相来.电针联合运动训练对脑梗死大鼠运动能力的影响及相关机制分析[J].中国康复医学杂志,2021,(7):779~786 |
| 电针联合运动训练对脑梗死大鼠运动能力的影响及相关机制分析 点此下载全文 |
| 赵晓红 严会荣 任 钰 牛相来 |
| 新疆维吾尔自治区中医医院康复理疗科，新疆维吾尔自治区乌鲁木齐市，830099 |
| 基金项目：新疆维吾尔自治区自然科学基金资助项目（2017D01C307） |
| DOI：10.3969/j.issn.1001-1242.2021.07.003 |
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| 摘要： |
| 摘要
目的：观察电针联合运动训练对脑梗死大鼠运动能力的影响，并探讨其对Wnt/β-catenin信号通路的影响。
方法：将大鼠随机分为假手术组（SC）、模型组（IC）、跑台组(T)、跑台+电针组(T+EA)、跑台+电针非穴位组(T+ENA)，各18只，SC组及IC组抓取后不予任何治疗。T组术后24h予匀速跑台训练，运动强度为10m/min，每天运动30min，每天1次，T+EA组在电针曲池、足三里穴后再予跑台训练，方法同跑台组，T+ENA组在电针非经穴点后再予跑台训练，方法同跑台组，各组均干预7天。观察各组大鼠神经行为学评分、步态、行为活动、脑梗死体积、病理学结构变化、脑细胞凋亡率，Western Blot及RT-PCR法检测脑组织Wnt-1/β-catenin信号通路关键分子蛋白及mRNA表达变化。
结果：神经行为学检测显示T+EA组脑梗死大鼠的神经缺损评分下调，大鼠的步态及行为活动改善。TTC染色、HE染色、透射电镜显示与其他组相比，T+EA组大鼠的脑梗死体积明显缩小，病理学形态及超微结构明显改善，Western Blot及RT-PCR显示T+EA可明显上调大鼠脑组织中Wnt-1、β-catenin、Bcl-2的蛋白及mRNA水平，下调GSK-3β、Bax的蛋白及mRNA水平（P＜0.05）。
结论：电针联合运动训练可明显改善脑梗死大鼠运动能力，其作用机制可能与激活Wnt/β-catenin信号通路有关。 |
| 关键词：脑梗死 运动能力 电针 运动训练 Wnt/β-catenin信号通路 |
| Effects of electroacupuncture combined with exercise training on exercise ability of rats with cerebral infarction and analysis of related mechanism Download Fulltext |
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| Xinjiang Uygur Autonomous Region Hospital of Traditional Chinese Medicine Rehabilitation Physiotherapy，Xinjiang ，Urumqi，830099 |
| Fund Project: |
| Abstract: |
| Abstract
Objective： To observe the effect of electroacupuncture combined with exercise training on motor ability of rats with cerebral infarction and to explore its effect on Wnt/ β-catenin signal pathway.
Method： Rats were randomly divided into sham operation group (SC)， model group (IC)， treadmill group (T)， treadmill plus electroacupuncture group (T+EA) and treadmill plus electroacupuncture non-acupoint group (T+ENA) with 18 rats in each group. SC group and IC group did not receive any treatment. T group was given uniform treadmill training 24 hours after operation， and exercise intensity was 10m/min， 30 minutes a day， once a day. T+EA group was given treadmill training after electroacupuncture at Quchi and Zusanli points. The method was the same as treadmill group， and T+ENA group was given treadmill training after electroacupuncture at non-acupoints. All groups were intervened for 7 days. The changes of neurobehavior， gait， activities，cerebral infarction volume， pathological structure， apoptosis rate of brain cells， and the expression of key molecular proteins and mRNA of Wnt-1/β-catenin signal pathway in brain tissue were detected by Western Blot and RT-PCR.
Result： Neuroethology showed that T+EA could down-regulate the neurological deficit score and improve the gait and activities of rats with cerebral infarction. TTC staining， HE staining and transmission electron microscope showed that the volume of cerebral infarction in T+EA group was significantly smaller than that in other groups. Pathological morphology and ultrastructure significantly improved， Western Blot and RT-PCR showed that T+EA could significantly up-regulate the protein and mRNA， of Wnt-1， β-catenin， Bcl-2 and mRNA， in brain tissue of rats， and down-regulate the protein and mRNA levels of GSK-3 β， Bax（P＜0.05）.
Conclusion： Electroacupuncture combined with exercise training can significantly improve the motor ability of rats with cerebral infarction，and its mechanism may be related to the activation of Wnt/β-catenin signal pathway. |
| Keywords:cerebral infarction motor ability electroacupuncture exercise training Wnt/β-catenin signal pathway |
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