| 邓 畅,陈丹凤,仇蓉蓉,张 泓,邹莹洁,徐雅钰,熊 丹,谭 洁.有氧运动训练对血管性痴呆大鼠肠道菌群结构的影响[J].中国康复医学杂志,2022,(4):443~450 |
| 有氧运动训练对血管性痴呆大鼠肠道菌群结构的影响 点此下载全文 |
| 邓 畅 陈丹凤 仇蓉蓉 张 泓 邹莹洁 徐雅钰 熊 丹 谭 洁 |
| 湖南中医药大学针灸推拿与康复学院，湖南省长沙市，410208 |
| 基金项目：湖南省自然科学基金项目（2018JJ3384）；湖南省中医药科研计划项目（2021233)；湖南中医药大学医学技术一流学科开放基金项目（2018YXJS03，2021YXJS01） |
| DOI：10.3969/j.issn.1001-1242.2022.04.002 |
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| 摘要： |
| 摘要
目的：探讨有氧运动训练治疗对血管性痴呆大鼠肠道菌群结构的影响。
方法：选择6周龄SPF级SD雄性大鼠，使用双侧颈总动脉永久性结扎法制备血管性痴呆大鼠模型，成模后随机分为模型组（Model）、有氧运动组（Swim），另取一组为假手术组（Sham），仅分离颈总动脉不结扎），每组12只。造模4周后进行干预，Swim组予以每日不负重游泳训练20min，共4周。进行Morris水迷宫检测学习记忆能力、电镜观察海马CA1区神经元超微结构、HE染色检测结肠组织病理变化、16S rDNA测序法检测肠道菌群。
结果：与Sham组比较，干预后Model组平均逃避潜伏期明显延长（P＜0.01），穿越平台次数明显减少（P＜0.05）；与Model组比较，Swim组大鼠平均逃避潜伏期明显缩短（P＜0.01），穿越平台次数明显增加（P＜0.05）；主成分分析（PCA）结果显示三组间群落的物种差异性具有显著性（P＜0.05），在属分类水平上，Model组较Sham组Ruminococcus_1（瘤胃球菌属）丰度明显增加（P＜0.05）；Swim组较Model组Ruminococcus_1丰度明显减少（P＜0.05），Lactobacillus（乳酸杆菌属）丰度明显增加（P＜0.05）。
结论：有氧运动训练可调节血管性痴呆大鼠肠道菌群结构，改善其学习记忆功能。 |
| 关键词：有氧运动训练 血管性痴呆 肠道菌群 大鼠 |
| Effects of aerobic exercise training on intestinal flora constructures of rats with vascular dementia Download Fulltext |
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| Hunan University of Chinese Medicine，Changsha，Hunan，410208 |
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| Abstract: |
| Abstract
Objective： To explore the effects of aerobic exercise training on intestinal flora constructures of rats with vascular dementia.
Method： 6-week-old SPF SD male rats was included in this study and the model of vascular dementia was induced by permanent ligation of bilateral common carotid arteries. After the model was successfully established， the rats were randomly divided into model group, aerobic-exercise group and sham-operation group (carotid arteries only separated without ligation) ，with 12 rats in each group. After 4 weeks recovery， the intervention was carried out. The aerobic-exercise group was given non-weight-bearing swimming training for 20 minutes, each day for 4 weeks. Morris water maze was used to detect learning and memory ability， electron microscope was used to observe the ultrastructure of neurons in hippocampal CA1 region， HE staining was used to detect the pathological changes of colon tissue， and 16S rDNA sequencing was used to detect intestinal flora.
Result： Compared with the sham-operation group， the average escape latency of the model group was significantly prolonged (P<0.01)， and the times of crossing the platform was significantly decreased (P<0.05). Compared with model group， the average escape latency in aerobic-exercise group was significantly shortened (P<0.01)， and the number of crossing platform was significantly increased (P<0.05). The results of PCA showed that the species differences among the three groups were significantly different (P<0.05). At the level of genus classification， the abundance of Ruminococcus_1 in the model group was significantly increased compared with that in the sham operation group (P<0.05). Compared with model group， the abundance of Ruminococcus_1 in the aerobic exercise group was significantly decreased (P<0.05)， while the abundance of Lactobacillus was significantly increased (P<0.05).
Conclusion： Aerobic exercise training can regulate the structure of intestinal flora and improve the learning and memory function in rats with vascular dementia. |
| Keywords:aerobic exercise training vascular dementia intestinal flora the rat |
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