| 董晓阳,陈利薇,王子雯,刘军仕,冯 珍.迷走神经电刺激治疗对脑外伤意识障碍大鼠前额叶皮层NLRP3炎症小体表达变化的影响[J].中国康复医学杂志,2022,(5):587~593 |
| 迷走神经电刺激治疗对脑外伤意识障碍大鼠前额叶皮层NLRP3炎症小体表达变化的影响 点此下载全文 |
| 董晓阳 陈利薇 王子雯 刘军仕 冯 珍 |
| 南昌大学第一附属医院康复医学科，江西省南昌市，330006 |
| 基金项目：江西省自然科学基金青年项目（20202BAB216018）；江西省教育厅青年项目（GJJ190125）；江西省卫生健康委员会科技计划项目（20204202）；江西省中医药管理局科技计划项目（2019A117） |
| DOI：10.3969/j.issn.1001-1242.2022.05.003 |
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| 摘要
目的：研究前额叶皮层NLRP3（nucleotide-binding domain-like receptor protein 3）炎症小体在迷走神经电刺激（vagus nerve stimulation， VNS）对脑外伤（traumatic brain injury， TBI）意识障碍大鼠的促醒作用及相关机制。
方法：将30只SD大鼠随机分为3组：TBI组、TBI+VNS组、TBI+VNS+NLRP3组，建立TBI意识障碍大鼠模型，应用VNS刺激TBI意识障碍大鼠，通过意识状态行为学量表评估意识状态水平改变情况，并用Western-Blot、免疫组织化学技术、QPCR技术分别检测各组大鼠前额叶皮层NLRP3、ASC、Caspase-1、Bax、Bcl-2、IL-1β、IL-18的表达变化。
结果：TBI意识障碍大鼠给予VNS刺激后意识状态水平得到改善，且前额叶皮层组织中NLRP3、ASC、Caspase-1、Bax、IL-1β、IL-18的表达明显低于TBI组，Bcl-2高于TBI组；而给予NLRP3激动剂后大鼠意识状态水平下降，同时伴随前额叶皮层组织中ASC、Caspase-1、Bax、TNF-α的表达升高，Bcl-2表达降低。
结论：VNS改善TBI大鼠意识水平的作用机制之一可能是通过抑制前额叶皮层NLRP3炎症小体的表达，最终降低神经细胞炎症反应和抗凋亡反应达到神经保护作用。 |
| 关键词：迷走神经电刺激 脑外伤 意识障碍 NLRP3炎症小体 神经炎症 神经凋亡 |
| Vagus nerve stimulation down regulate the expression of nucleotide-binding domain-like receptor protein 3 inflammasome in prefrontal cortex of traumatic brain injury rats with disorders of consciousness Download Fulltext |
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| Department of Rehabilitation Medicine， the First Affiliated Hospital of Nanchang University，Nanchang， Jiangxi， 330006 |
| Fund Project: |
| Abstract: |
| Abstract
Objective： To explore effects of nucleotide-binding domain-like receptor protein 3 (NLRP3) inflammasome in vagus nerve stimulation (VNS) on promoting wakefulness of rats with traumatic brain injury (TBI) induced disorders of consciousness rats.
Method： A total of 30 Sprague-Dawley rats were randomly divided into three groups： TBI group， TBI+VNS group and TBI+VNS+NLRP3 group. TBI induced disorder of consciousness rat model was established， and VNS or VNS+NLRP3 agonist injection was used correspondingly to TBI rats with consciousness disorder. The level of consciousness was assessed by the State of Consciousness Behavioral Scale. Western-Blot， immunohistochemistry， and QPCR were used to detect the expression of NLRP3， ASC， Caspase-1， Bax， Bcl-2， IL-1β， IL-18 in the prefrontal cortex of each group of rats.
Result： Comparing with the TBI group, the level of consciousness was better, the expression of NLRP3， ASC， Caspase-1， Bax， IL-1β， and IL-18 in the prefrontal cortex were significantly lower， and Bc-2 was higher after VNS. Nevertheless, the level of consciousness in VNS+NLRP3 agonist injection group was decreased， and the expression of ASC， Caspase-1， Bax， and TNF-α in the prefrontal cortex were increased， while the expression of Bcl-2 was decreased.
Conclusion： One of the mechanism for VNS to improve the level of consciousness in TBI rats is to inhibit the expression of NLRP3 inflammasomes in the prefrontal cortex， thus ultimately reduce the inflammatory response and anti-apoptotic response of nerve cells to achieve neuroprotection. |
| Keywords:vagus nerve electrical stimulation traumatic brain injury disorder of consciousness NLRP3 inflammasome neuroinflammation neuroapoptosis |
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