| 张崇林,王 卉,丁孝民,刘绍生,王世香,夏 志.运动和高脂膳食对代谢综合征大鼠心肌细胞氧化应激反应的影响[J].中国康复医学杂志,2022,(8):1039~1045 |
| 运动和高脂膳食对代谢综合征大鼠心肌细胞氧化应激反应的影响 点此下载全文 |
| 张崇林 王 卉 丁孝民 刘绍生 王世香 夏 志 |
| 井冈山大学体育学院，江西省吉安市，343009 |
| 基金项目：江西省教育厅科技计划重点项目(GJJ170627)；江西省教育厅科技规划项目（GJJ180582） |
| DOI：10.3969/j.issn.1001-1242.2022.08.005 |
| 摘要点击次数: 706 |
| 全文下载次数: 366 |
| 摘要： |
| 摘要
目的：对代谢综合征（metabolism syndrome，MS）大鼠实施有氧运动和饮食干预，观察运动和高脂膳食对MS大鼠氧化应激反应（oxidative stress ，OS）的影响，探讨运动和饮食对MS大鼠心血管氧化应激作用的可能机制。
方法：70只雄性清洁级SD大鼠，随机抽取6只作为基础对照组（C），其余高脂高盐高糖饲养18周建立MS大鼠模型。MS模型大鼠随机分为普食安静（RC）、高脂安静（HC）、普食运动（RE）、高脂运动（HE）4组，每组6只。HE组和RE组行运动干预，HC组和HE组继续高脂饲料饲养，12周后同期处死。检测血清巨噬细胞趋化蛋白-1（MCP-1）、粘附因子纤溶酶原激活物抑制剂I（PAI-1）、氧化应激型氧化低密度脂蛋白（ox-LDL）、内皮型一氧化氮合酶（eNOS）水平；检测心肌组织MCP-1、PAI-1、ox-LDL、eNOS及过氧化物酶体增殖物激活受体α（PPARα）mRNA表达量。
结果：血清指标中，与C组比，HC与RC组ox-LDL、除RE组外MCP-1、HC组PAI-1、RC与RE组eNOS均显著升高（P＜0.01）；与HC组相比，HE与RE组ox-LDL和MCP-1、PAI-1所有组均显著降低（P＜0.05，P＜0.01）、eNOS RC与RE组显著升高（P＜0.05，P＜0.01）；与RC组相比，HE与RE组MCP-1均显著降低（P＜0.05，P＜0.01）；与HE组相比，RE组MCP-1显著降低（P＜0.01），eNOS显著升高（P＜0.01）。心肌组织中，与C组比，HC与RC组MCP-1、PAI-1显著升高，PPARα显著降低，RC、HE与RE组eNOS显著升高（P＜0.01）；与HC组相比，HE与RE组MCP-1、PAI-1及RC组PAI-1显著降低，HE与RE组eNOS、PPARα及RC组eNOS显著升高（P＜0.05，P＜0.01）；与RC组相比，RE组MCP-1、PAI-1显著降低，eNOS、PPARα显著升高（P＜0.05，P＜0.01）；与HE组相比，RE组MCP-1显著降低（P＜0.01），eNOS显著升高（P＜0.05）；PPARα与MCP-1和PAI-1呈负直线相关（P＜0.01）。
结论：MS大鼠氧化应激反应增强。运动和饮食干预均能降低MS大鼠心肌氧化应激反应。运动能提高氧化应激调节因子PPARα mRNA表达而饮食控制作用不大，PPARα mRNA表达增加进而调控MCP-1，PAI-1，eNOS mRNA的表达，可能是有氧运动抗氧化应激的机制。 |
| 关键词：有氧运动 高脂膳食 代谢综合征 氧化应激 |
| Effects of exercise and high-fat dietary intervention on myocardial oxidative stress in male rats with metabolism syndrome Download Fulltext |
|
| College of Physical Education， Jinggangshan University，Ji'an，Jiangxi， 343009 |
| Fund Project: |
| Abstract: |
| Abstract
Objective：Both aerobic exercise and dietary control could relieve . The objective of present study is to observe the effects of aerobic exercise and dietary control on oxidative stress (OS) in rats with metabolism syndrome(MS) and explore the underlying mechanisms.
Method： In this study， a total of 70 rats were used. 6 of them were randomly assigned to the control group and the rest were subjected to establish MS model by high-fat diet for 18wk. Subsequently， the MS model rats were divided into HC(model control with high-fat diet)，RC(model control with routine diet)，HE(model training with high-fat diet) and RE(model training with routine diet) group for 12wk. Macrophage chemoattractant protein-1(MCP-1)，plasminogen activator inhibitor I(PAI-1)， oxidized low density lipoprotein (ox-LDL)，and endothelial nitric oxide synthase(eNOS) were tested in serum，and the expression of MCP-1，PAI-1， eNOS and regulatory factor of OS Peroxisome proliferators-activated receptors-alpha(PPARα) were detected in myocardium.
Result： The data of markers of OS in serum showed that aerobic exercise and alimentary control have the effect of anti-oxidative stress in different way. The expression of many genes of OS were changed in aerobic exercise and alimentary control. PPARαthat is a regulatory factor for OS had a significant increase by aerobic exercise but not by diet control. PPARα was negatively correlated with MCP-1 and PAI-1.
Conclusion： OS increased in MS rats. Aerobic exercise and alimentary control could decrease the OS and reduce the damage in MS rats. PPARα, the OS regulatory factor, was just enhanced by aerobic exercise but not by diet control.The possible mechanism of exercise-induced anti-oxidation stress was to enhance the expression of PPARα which mediated the expression of OS related genes such as MCP-1，PAI-1，eNOS in cardiovascular. |
| Keywords:aerobic exercise high-fat dietary metabolic syndrome oxidative stress |
|
| 查看全文 查看/发表评论 |
