| 李凤仪,张浴尘,鹿国晖,石丽君,吴 迎.有氧运动对自发性高血压大鼠心肌MG53/NF-κB信号通路的影响[J].中国康复医学杂志,2022,(9):1165~1173 |
| 有氧运动对自发性高血压大鼠心肌MG53/NF-κB信号通路的影响 点此下载全文 |
| 李凤仪 张浴尘 鹿国晖 石丽君 吴 迎 |
| 北京体育大学运动与体质健康教育部重点实验室，北京市，100084 |
| 基金项目：国家自然科学基金项目（31771312）；中央高校基本科研业务费专项资金资助项目（2020054） |
| DOI：10.3969/j.issn.1001-1242.2022.09.003 |
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| 摘要
目的：研究有氧运动对自发性高血压大鼠心脏形态与功能的影响，并探讨MG53/NF-κB信号通路在有氧运动调控高血压心脏重塑中的可能作用，为高心病的防治提供实验依据。
方法：正常血压对照大鼠（Wistar-Kyoto rat， WKY）随机分为正常血压运动组（WKY-EX）和正常血压安静组（WKY-SED）；自发性高血压大鼠（spontaneously hypertensive rat， SHR）随机分为高血压运动组（SHR-EX）和高血压安静组（SHR-SED）；每组12只；3月龄雄性。经12周跑台训练（坡度0°，20m/min，5d/w），测量尾动脉血压；Millar压力-容积系统测定心功能；WGA染色分析心肌细胞横截面积；Masson染色分析心肌胶原容积分数（CVF%）；实时荧光定量PCR测定心钠肽（atrial natriuretic peptide， ANP）、脑钠肽（brain natriuretic peptide， BNP）、MG53和NF-κB p65 mRNA相对表达量；蛋白免疫印迹测定心肌MG53、NF-κB p65和p-NF-κB p65蛋白表达。
结果：①12周有氧运动可有效降低SHR的心率和收缩压；②SHR-SED组CVF%和心肌细胞横截面积显著高于WKY-SED组（P<0.01），SHR-EX组CVF%显著低于SHR-SED组（P<0.01），心肌细胞横截面积无显著性差异（P>0.05）；③与WKY-SED组相比，SHR-SED组射血分数（EF）、左心室内压上升最大速率（+dp/dtmax）和左心室内压下降最大速率（-dp/dtmax）绝对值均显著下降（P<0.01），与SHR-SED组相比，SHR-EX组EF（P<0.05）、+dp/dtmax（P<0.05）和-dp/dtmax绝对值（P<0.01）均显著增高；④与WKY-SED组相比，SHR-SED组心肌ANP和BNP mRNA表达量显著增加（P<0.01），NF-κB p65 mRNA表达量也显著增加（P<0.05），MG53 mRNA表达量无显著性差异（P>0.05）；与SHR-SED组相比，SHR-EX组心肌ANP、BNP和NF-κB p65 mRNA表达量显著降低（P<0.01），MG53 mRNA表达量显著增加（P<0.01）；⑤与WKY-SED组相比，SHR-SED组心肌MG53蛋白表达水平无显著性差异（P>0.05），NF-κB p65蛋白相对磷酸化水平显著升高（P<0.01），与SHR-SED组相比，SHR-EX组心肌MG53蛋白表达水平显著升高（P<0.01），NF-κB p65蛋白相对磷酸化水平显著下降（P<0.01）。
结论：有氧运动可能通过上调MG53抑制NF-κB表达，从而改善高血压心脏功能，延缓高血压性心脏病的发生发展。 |
| 关键词：MG53 有氧运动 高血压 心功能 |
| Effects of aerobic exercise on MG53/NF-κB signaling pathway in the myocardium of spontaneously hypertensive rats Download Fulltext |
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| Beijing Sport University， Beijing， 100084 |
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| Abstract: |
| Abstract
Objective： To investigate the possible role of MG53/NF-κB signaling pathway in the regulation of hypertensive cardiac remodeling by aerobic exercise， and to provide a new idea for the treatment of hypertensive heart disease by combining aerobic exercise with drugs.
Method： Twenty-week-old male SHR and WKY rats were randomly assigned to sedentary and exercise training groups， respectively， 12 rats in each group. After 12 weeks of running platform training （incline 0°， 20m/min， 5d/w）， tail artery blood pressure was measured； Millar pressure-volume system was used to determine cardiac function； WGA staining was used to analyze the cell surface area of cardiomyocytes； masson staining was used to analyze myocardial collagen volume fraction（CVF%）； real-time fluorescence quantitative PCR was performed to determine the relative expression of cardiac natriuretic peptide（ANP）， brain natriuretic peptide （BNP）， MG53 and NF-κB p65 mRNA； western blot measures the protein expression of MG53， NF-κB p65 and p-NF-κB p65.
Result： ①Twenty weeks aerobic exercise effectively reduced heart rate and systolic blood pressure in the hypertensive rats. ②CVF% and cardiac myocyte cross-sectional area were significantly higher in the SHR-SED group than in the WKY-SED group（P<0.01）. CVF% was significantly lower in the SHR-EX group than in the SHR-SED group（P<0.01）. There was no significant difference in cardiomyocyte cross-sectional area（P>0.05）. ③Compared with the WKY-SED group， the SHR-SED group showed significantly lower EF， +dp/dtmax and -dp/dtmax in absolute terms（P<0.01）. Compared with the SHR-SED group， the SHR-EX group showed significantly higher EF （P<0.05）， +dp/dtmax（P<0.05）， and -dp/dtmax（P<0.01） in absolute terms. ④Compared with the WKY-SED group， myocardial ANP and BNP mRNA expressions were significantly increased in the SHR-SED group （P<0.01）， and NF-κB p65 mRNA expression was also significantly increased（P<0.05）， while MG53 mRNA expression had no significant difference（P>0.05）. Compared with those in the SHR-SED group， myocardial ANP， BNP and NF-κB p65 mRNA expression was significantly lower（P<0.01） and MG53 mRNA expression was significantly higher（P<0.01） in the SHR-EX group. ⑤Compared with the WKY-SED group， myocardial MG53 protein expression level had no change in the SHR-SED group（P>0.05） and NF-κB p65 protein phosphorylation level was significantly increased in the SHR-EX group（P<0.01） and compared with SHR-SED group， myocardial MG53 protein expression level was significantly increased in the SHR-EX group（P<0.01） and NF-κB p65 protein phosphorylation level was significantly decreased（P<0.01）.
Conclusion： Aerobic exercise may inhibit NF-κB expression through up regulation of MG53， thereby improving hypertensive cardiac function and delaying the development of hypertensive heart disease. |
| Keywords:MG53 aerobic exercise hypertension cardiac function |
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