| 赵海涛,曹庆雷,邓中原.PGC-1α启动子甲基化介导在热大鼠预适应延缓运动疲劳中的机制研究[J].中国康复医学杂志,2022,(10):1306~1311 |
| PGC-1α启动子甲基化介导在热大鼠预适应延缓运动疲劳中的机制研究 点此下载全文 |
| 赵海涛 曹庆雷 邓中原 |
| 北京科技大学体育部，北京，100083 |
| 基金项目： |
| DOI：10.3969/j.issn.1001-1242.2022.10.003 |
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| 摘要： |
| 摘要
目的：探讨热预适应通过调节过氧化物酶体增殖物活化受体γ协同刺激因子-1α（peroxisome proliferator-activated receptor-γ coactivator-1α，PGC-1α）基因的启动子甲基化修饰从而延缓运动疲劳发生的机制。
方法：选取12只8周龄雄性SD大鼠随机分成热预适应组（n=6）与正常对照组（n=6），热预适应组经过42℃热应激处理15min，每天间隔处理3次，连续处理7d，结束后每组取3只SD大鼠采集肌肉组织进行启动子甲基化检测，随后利用生物信息学软件预测SD大鼠肌肉组织中PGC-1α基因的CpG岛，针对CpG岛设计检测引物，采用重亚硫酸盐的测序法分析肌肉组织内PGC-1α基因启动子区CpG岛的甲基化程度，同时采用Western Blot法检测PGC-1α蛋白表达情况。将每组的另3只大鼠进行跑台一次性力竭运动，记录力竭时间。
结果：热预适应处理显著延缓了运动疲劳的发生。同时，与对照组PGC-1α基因启动子区域CpG岛高度甲基化（50.20%±3.13%或48.78%±3.38%）相比，热预适应处理显著降低了PGC-1α基因的甲基化（18.57%±2.12%或19.80%±1.99%），但热预适应组PGC-1α基因的蛋白表达量却显著高于对照组。因此，SD大鼠在1w的热预适应过程中PGC-1α基因启动子区CpG岛的甲基化模式发生了显著变化，即随着热预适应处理时间的增加，PGC-1α基因启动子区甲基化程度逐渐降低，但PGC-1α基因的表达量显著升高。
结论：对大鼠进行热预适应处理可以通过降低PGC-1α启动子的甲基化水平增加PGC-1α基因的表达量，这可能是热预适应通过PGC-1α基因启动子甲基化水平的改变延缓运动疲劳发生的机制之一。 |
| 关键词：过氧化物酶体增殖物活化受体γ协同刺激因子-1α 运动疲劳 热预适应 CpG岛 甲基化 |
| Study on the mechanism of PGC-1 α promoter methylation mediated by thermal pre adaptation in delaying exercise fatigue Download Fulltext |
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| Dept. of Physical Education，University of Science and Technology Beijing，100083 |
| Fund Project: |
| Abstract: |
| Abstract
Objective： To investigate the mechanism of heat pre-adaptation-induce delaying of exercise fatigue by regulating the promoter methylation modification of peroxisomeproliferator-activated receptor-γ coactivator-1α（PGC-1α）.
Method： Twelve male Sprague Dawley rats （SD rats） of 8 weeks old were randomly divided into two groups： the heat pre-adaptation group （n=6） and the normal control group （n=6）. The heat pre-adaptation group was treated with heat shock at 42℃ for 15min， 3 times a day， and 7 days continuously. After that，muscle tissue was collected in 3 rats of each group for promoter methylation detection， and then the PGC-1α gene in the muscle tissue was predicted by bioinformatics software. The methylation degree of CpG island in the promoter region of PGC-1α gene was analyzed by bisulfite sequencing， and the change of PGC-1α protein content was detected by Western Blot. The other three rats in each group ran on the treadmill and the exhaustion time was recorded.
Result： The heat pre-adaptation significantly delayed the occurrence of exercise fatigue. At the same time， the results showed that the heat pre-adaptation significantly reduced the methylation of PGC-1α gene （18.57%±2.12% or 19.80%±1.99%）, when compared that in the the control group （50.20%±3.13% or 48.78%±3.38%）. However，the protein expression of PGC-1α gene in the heat pre-adaptation group was significantly higher than that in the control group. Therefore，the methylation pattern of CpG island in PGC-1α gene promoter region changed significantly during one week of heat pre-adaptation. With the increase of heat pre-adaptation time， the methylation degree of PGC-1α gene promoter region decreased gradually，while the expression of PGC-1α gene increased significantly.
Conclusion： These results indicate that heat pre-adaptation can increase the expression of PGC-1α gene by reducing the methylation level of PGC-1α promoter， which may be one of the mechanisms of heat pre-adaptation delaying the occurrence of exercise fatigue by changing the methylation level of PGC-1α promoter. |
| Keywords:peroxisome proliferator-activated receptor gamma-costimulatory factor-1α exercise fatigue thermal preconditioning CpG island methylation |
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