| 李保龙,梁润玉,裴 飞,张金朋,钱 铖,朱路文.基于缝隙连接蛋白-43调控的运动预处理促进大鼠脑缺血再灌注后神经血管单元重构的机制研究[J].中国康复医学杂志,2023,(3):299~304 |
| 基于缝隙连接蛋白-43调控的运动预处理促进大鼠脑缺血再灌注后神经血管单元重构的机制研究 点此下载全文 |
| 李保龙 梁润玉 裴 飞 张金朋 钱 铖 朱路文 |
| 黑龙江中医药大学附属第二医院，黑龙江省哈尔滨市，150000 |
| 基金项目：孙申田青年人才基金（KY-201803）；中国博士后科学基金（2017M611411） |
| DOI：10.3969/j.issn.1001-1242.2023.03.003 |
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| 摘要： |
| 摘要
目的：探讨运动预处理通过影响缝隙连接蛋白-43（Cx43），促进脑缺血再灌注损伤（CIRI）后血管神经单元重构的作用。
方法：将72只雄性SD大鼠分为假手术组、模型组、运动预处理组，并用线栓法制作大脑中动脉缺血/再灌注模型。在恢复灌注的1天、3天后分别进行神经功能缺损评分、Western Blot及免疫荧光染色检测Cx43蛋白表达水平、免疫组化法检测CD34表达水平。
结果：与假手术组相比，模型组神经功能缺损评分、缺血侧皮层脑组织中Cx43蛋白表达水平显著升高（P<0.01），CD34细胞阳性率显著下降（P<0.01）。与模型组相比，运动预处理组神经功能缺损评分、缺血侧皮层脑组织中Cx43蛋白表达水平较低，CD34细胞阳性率上升，差异均有显著性意义（P<0.01）。
结论：作为CIRI和神经血管单元重构机制靶点之一，基于Cx43调控的运动预处理可通过抑制CIRI后Cx43的激活，上调CD34表达，发挥作用。 |
| 关键词：脑缺血再灌注 运动预处理 神经血管重构 缝隙连接蛋白-43 CD34 |
| Mechanism of exercise preconditioning based on Cx43 promoting neurovascular unit remodeling after cerebral ischemia-reperfusion in rats Download Fulltext |
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| The Second Affiliated Hospital of Heilongjiang University of Traditional Chinese Medicine，150000 |
| Fund Project: |
| Abstract: |
| Abstract
Objective： To investigate the effect of exercise preconditioning on the remodeling of vascular nerve units after cerebral ischemia reperfusion injury（CIRI） by affecting connexin-43 （Cx43）.
Method： Seventy-two male SD rats were divided into sham group， model group and exercise preconditioning group. Middle cerebral artery occlusion/reperfusion model was made by thread embolus. Modified neurologic severity score， Western Blot and Immunofluorescence staining were used to detect the expression of Cx43 protein and Immunohistochemistry was used to detect the expression of CD34 at 1 or 3 day after reperfusion.
Result： Compared with the sham group， the modified neurologic severity score and the expression of Cx43 in the ischemic cortex of the model group increased significantly （P<0.01）， and the positive rate of CD34 cells decreased significantly （P<0.01）. Compared with the model group， the modified neurologic severity score， the expression of Cx43 protein in the ischemic cortex were lower， and the positive rate of CD34 cells in the exercise preconditioning group was higher（P<0.01）.
Conclusion： As one of the targets of CIRI and neurovascular unit remodeling mechanism， exercise preconditioning based on Cx43 can play a role by inhibiting the activation of Cx43 after CIRI and up-regulating the expression of CD34 after CIRI. |
| Keywords:cerebral ischemia-reperfusion exercise preconditioning neurovascular remodeling connexin-43 CD34 |
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