| 王 浩,刘海潮,陈少清,李 明,郭明玲,王诗忠,林建平.骨骼肌钝挫伤大鼠模型的构建与评价[J].中国康复医学杂志,2024,(1):24~30 |
| 骨骼肌钝挫伤大鼠模型的构建与评价 点此下载全文 |
| 王 浩 刘海潮 陈少清 李 明 郭明玲 王诗忠 林建平 |
| 福建医科大学健康学院，福建省福州市，350122 |
| 基金项目：国家自然科学基金项目（81904317，81973924）；福建省自然科学基金项目（2019J01360，2021J01817） |
| DOI：10.3969/j.issn.1001-1242.2024.01.004 |
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| 摘要： |
| 摘要
目的：建立可靠、稳定、符合临床实际的大鼠骨骼肌钝挫伤模型。
方法：通过170g重物，70cm高度，用底部直径0.5cm弹簧缓冲聚合式打击部，以引导下落的撞击方法，连打5次，建立大鼠腓肠肌钝挫伤模型。分别于造模后0h、12h、24h、3d、7d、14d，采用CatWalk分析系统评估大鼠步态改变；HE染色观察大鼠局部组织病理学改变，透射电子显微镜观察局部组织超微结构的改变。酶联免疫吸附试验（enzyme linked immunosorbent assay，ELISA）检测局部组织肿瘤坏死因子（tumor necrosis factor-α， TNF-α）含量变化。
结果：①CatWalk步态参数：与空白组相比，模型组的平均速度与最大速度变化率均明显下降，差异具有显著性意义（P<0.001）；与健康侧肢体组相比：受伤侧肢体组肢体的最大接触面积、足印面积和站立相持续时间均明显减少，差异具有显著性意义（P<0.05）。②HE染色：观察到3d后大量炎症细胞浸润，肌纤维大量溶解坏死，14d尽管瘢痕组织仍然存在，但HE染色上的组织修复与空白组几乎没有区别。③超微结构：打击后0h部分肌浆网出现空泡现象，3d肌纤维和部分肌浆网溶解，炎症反应加重，到14d基本恢复正常。④TNF-α表达水平：0h开始至12h这段时间内，TNF-α含量即开始逐渐升高达到高峰，并在损伤后12—72h一直保持较高水平，与空白组相比差异具有显著性意义（P<0.05），到14d基本恢复正常水平。
结论：以锤重170g、70cm高度、底部带弹簧缓冲装置的直径0.5cm圆钝型打击头，连打5次，可对大鼠骨骼肌造成中度损伤，其自然恢复时间至少14d，适用于大鼠骨骼肌钝挫伤模型的建立，可为今后大鼠骨骼肌钝挫伤模型提供参考依据。 |
| 关键词：骨骼肌钝挫伤 动物模型 步态分析 超微结构 肿瘤坏死因子 |
| Construction and evaluation of a rat model with blunt contusion of skeletal muscle Download Fulltext |
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| Department of Rehabilitation Medicine， School of Health， Fujian Medical University， Fuzhou， 350122 |
| Fund Project: |
| Abstract: |
| Abstract
Objective： To establish a reliable，stable and clinically realistic model of blunt contusion injury to the rat skeletal muscle.
Method： A model of the rat gastrocnemius muscle blunt contusion injury was established by using a weight of 170g，a height of 70cm and a spring with a diameter of 0.5cm at the bottom to buffer the polymeric striking part and guide the fall to hit for 5 consecutive times. The gait changes of rats were evaluated by CatWalk analysis system at 0h， 12h， 24h， 3 days， 7 days and 14 days after modeling； local histopathological changes were observed by HE staining and local ultrastructural changes were observed by transmission electron microscopy. Enzyme-linked immunosorbent assay （ELISA） was used to detect the changes of tumor necrosis factor-α （TNF-α） in local tissues.
Result： Compared with the blank group， the mean speed and maximum speed change rate of the model group were significantly decreased， and the difference was statistically significant （P<0.001）； compared with the normal foot group, the maximum contact area， footprint area and standing phase duration of the limb of the injured limb group were significantly decreased， and the difference was statistically significant （P<0.05）. A large infiltration of inflammatory cells and massive lysis necrosis of muscle fibers were observed on HE staining after 3 days. By 14 days，although scar tissue was still present， tissue repair with HE staining was almost indistinguishable from that of the blank group. Vacuolation was observed in part of the sarcoplasmic reticulum at 0h after the blow， and lysis of muscle fibers and part of the sarcoplasmic reticulum at 3 days with increased inflammatory response which basically returned to normal by 14 days. The TNF-α level began to rise gradually and reached a peak from 0h to 12h after injury，and remained higher level from 12h to 72h， and the difference was statistically significant compared with the blank group （P<0.05），and basically returned to the normal level by 14 days.
Conclusion： The model can cause moderate damage of skeletal muscle in rats, with a 14-day natural recovery time at least. It can provide as one of the methods for for the establishment of the rat skeletal muscle blunt contusion model. |
| Keywords:skeletal muscle contusions animal model gait analysis ultrastructure tumor necrosis factor-α |
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