| 曹师承,孙黎光,赵 刚,刘慧莉,张 合.有氧运动对大鼠骨骼肌mTOR活性与蛋白表达的影响[J].中国康复医学杂志,2008,23(1):34~36 |
| 有氧运动对大鼠骨骼肌mTOR活性与蛋白表达的影响 点此下载全文 |
| 曹师承 孙黎光 赵 刚 刘慧莉 张 合 |
| 中国医科大学基础医学院生物化学与分子生物学教研室，沈阳，110001;中国医科大学基础医学院生物化学与分子生物学教研室，沈阳，110001 |
| 基金项目：辽宁省教育厅高等学校科研基金资助项目(2004D244) |
| DOI： |
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| 摘要： |
| 目的：观察有氧运动对大鼠骨骼肌哺乳动物雷帕霉素靶蛋白(mTOR)蛋白总量（t-mTOR）及磷酸化mTOR(p-mTOR)与磷酸化的核糖体蛋白S6激酶 (p-P70S6K)的影响。方法：SD大鼠随机分为对照组和运动组。运动组1.5 h/d训练，共7周。运动结束后分别在24h或48h取材，测定葡萄糖和胰岛素浓度；Western blot法检测骨骼肌t-mTOR蛋白表达、p-mTOR和p-P70S6K磷酸化水平。结果：与对照组比较，运动组胰岛素浓度降低；各运动组t-mTOR升高；p-mTOR和p-P70S6K活性增高显著。结论：运动能提高大鼠骨骼肌对胰岛素的敏感性，改善mTOR与P70S6K磷酸化及mTOR蛋白表达。 |
| 关键词：哺乳动物雷帕霉素靶蛋白 运动 骨骼肌 核糖体蛋白S6激酶 |
| Effects of aerobic exercises on mTOR phosphorylation and protein expression in skeletal muscle of rats Download Fulltext |
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| Department of Biochemistry and Molecular Biology, Shenyang, 110001;Department of Biochemistry and Molecular Biology, Shenyang, 110001 |
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| Abstract: |
| Objective:To investigate the effects of exercises on mTOR and P70S6K phosphorylation and protein expression of mTOR. Method: Male SD rats were randomly divided into control group and trained group. The trained rats were submitted to 1.5h of exercises daily and had a fragment of their excised gastrocnemius muscle, 24h or 48h after the last training session. The training lasted for 7 weeks.The changes in the expressions of mTOR and p-mTOR and p-P70S6K were determined by Western blotting. The serum levels of glucose and insulin were measured. Result: Glucose tolerance test diplayed blood insulin concentration decreased with exercises training. Exercises led to a marked increase in p-mTOR and p-P70S6K of trained group as compared with that of controls, and increased mTOR protein expression of training 1.5h/d, 24h and 48h after the last training session.Conclusion:Exercises could elevate skeletal muscle responsiveness to insulin, improve the p-mTOR and p-P70S6K and protein expression of mTOR. |
| Keywords:mammalian target of rapamycin exercises skeletal muscle ribosomal protein S6 kinas |
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