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于利人,周 蔚,陈立军,靳秋月,史 娜.大鼠脑组织总超氧化物歧化酶、锰超氧化物歧化酶和铜锌超氧化物歧化酶活性在不同训练条件下的变化[J].中国康复医学杂志,2011,26(6):546~549
大鼠脑组织总超氧化物歧化酶、锰超氧化物歧化酶和铜锌超氧化物歧化酶活性在不同训练条件下的变化    点此下载全文
于利人  周 蔚  陈立军  靳秋月  史 娜
武警医学院训练部临床管理处,天津,300162
基金项目:军队科研项目(WKH2009Z02)
DOI:
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摘要:
      摘要 目的:通过建立SD大鼠跑台运动模型,观察不同训练负荷条件下大鼠脑组织总超氧化物歧化酶(T-SOD),锰超氧化物歧化酶(Mn-SOD)和铜锌超氧化物歧化酶(CuZn-SOD)活性的变化。 方法:实验建立有氧、无氧、有氧和无氧交替运动SD大鼠跑台运动训练模型,有氧运动组采用递增负荷训练,无氧运动组采用高速间歇训练,交替运动组采用有氧运动与无氧运动交替训练,每组24只大鼠,均训练6周,并设立正常对照组(8只大鼠)。各组大鼠每训练2周处死8只,然后用机器匀浆法提取大鼠脑组织匀浆介质,可见分光光度计检测大鼠脑组织T-SOD,Mn-SOD和CuZn-SOD活性。 结果:训练2周后,无氧组大鼠脑组织T-SOD水平低于正常对照组(P<0.05),但随着训练周期的延长,其T-SOD水平逐渐增加(P<0.05);交替组大鼠脑组织T-SOD和Mn-SOD活性在训练2周时最高(P<0.05),之后随着训练周期的延长逐渐降低;各运动大鼠脑组织CuZn-SOD活性与正常对照组相比并无显著差异(P>0.05)。 结论:无氧运动训练一定时间后可以增加大鼠脑组织T-SOD的活性,交替运动大鼠训练2周对提高脑组织T-SOD和Mn-SOD活性最佳,运动对大鼠脑组织CuZn-SOD活性并无显著影响。
关键词:跑台运动  大鼠  脑组织  超氧化物歧化酶
Changes of activities of total-superoxide dismutase, manganase-superoxide dismutase and copper zinc-superoxide dismutase in rat’s brain at different training intensities    Download Fulltext
Department of Clinical Management, Department of Biochemistry, Armed Police Medical College, Tianjin, 300162
Fund Project:
Abstract:
      Abstract Objective: To observe the changes of activities of total-superoxide dismutase (T-SOD), manganese-superoxide dismutase (Mn-SOD) and copper zinc-superoxide dismutase (CuZn-SOD) in rat's brain via constructing motion models of aerobic exercise, anaerobic exercise as well as aerobic and anaerobic alternative treadmill exercise training. Method: Aerobic exercise group was constructed by progressive load exercise, anaerobic exercise group treated with high-speed interval training, alternative exercise group treated with aerobic exercise and anaerobic exercise alternatively(24 rats in each group, training for 6 weeks). Meanwhile, normal control group was established(8 rats). After every 2 weeks training 8 rats were sacrificed in each group. Activities of T-SOD, Mn-SOD and CuZn-SOD in rat's brain were measured. Result: Content of T-SOD in rat's brain in anaerobic exercise group was less than that in normal control group after training two weeks(P<0.05). However, with extension of training periods, the level of T-SOD increased gradually(P<0.05). After two weeks training activities of T-SOD and Mn-SOD in rat's brain in alternative training group were the highest(P<0.05), then it decreased following extension of training periods. There was no significant difference of activities of CuZn-SOD in rat's brain among exercise groups and normal control group(P>0.05). Conclusion: Anaerobic exercise for a certain period can increase the activity of T-SOD in rat's brain. Alternative training for two weeks may be the best for increasing the activities of T-SOD and Mn-SOD in rat's brain. This experiment suggested that exercise would not markedly influence the activity of CuZn-SOD in rat's brain.
Keywords:treadmill exercise  rat  brain tissue  superoxide dismutase
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