| 蔡 琛,洪营东,吴俊梅.低频电针对海洛因依赖小鼠海马N-甲基-D-天冬氨酸受体NR1、NR2A、NR2B亚型蛋白表达的影响[J].中国康复医学杂志,2012,27(4):324~329 |
| 低频电针对海洛因依赖小鼠海马N-甲基-D-天冬氨酸受体NR1、NR2A、NR2B亚型蛋白表达的影响 点此下载全文 |
| 蔡 琛 洪营东 吴俊梅 |
| 郑州人民医院康复科，郑州，郑州黄河路33号，450003 |
| 基金项目：青年科学基金项目（30300460） |
| DOI： |
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| 摘要
目的：通过观察低频电针对海洛因心理依赖小鼠海马N-甲基-D-天冬氨酸(NMDA)的亚型NMDAR1(NR1)、NMDAR-2A(NR2A)、NMDAR-2B(NR2B)表达的影响，探讨低频电针对海洛因成瘾记忆干预作用的可能中枢机制。
方法：筛选无天然位置偏爱的雄性小鼠45只，随机分为对照组、海洛因心理依赖模型组（模型组）、模型电针组（模针组），每组15只。海洛因剂量递增注射结合条件性位置偏爱（conditioned place preference, CPP）训练制作海洛因心理依赖模型，2Hz低频电针“内关”、“三阴交”，点刺“四神聪”穴。采用白箱CPP值变化评价低频电针治疗效应，免疫组化方法检测海马NMDA受体亚型（NR1、NR2A、NR2B）的表达。
结果：①模针组白箱CPP值较模型组明显降低(P＜0.05)。②模针组小鼠NR1、NR2B阳性表达较模型组增强(P＜0.05)。③模针组小鼠NR2A阳性表达较模型组降低(P＜0.05)。
结论：低频电针疗法能增强海洛因心理依赖小鼠海马NR1、NR2B，降低NR2A的阳性表达。这种改变可能与低频电针调节海洛因依赖的机制有关。 |
| 关键词：海洛因依赖 海马 低频电针 N-甲基-D-天冬氨酸 |
| The effect of electro-acupuncture with low frequency current on expressions of N-methyl-D-aspartate receptor subtypes NR1, NR2A, NR2B in hippocampus of heroin psychological dependent mice Download Fulltext |
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| Dept. of Rehabilitation Medicine, Zhengzhou People's Hospital, Zhengzhou, 450003 |
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| Abstract: |
| Abstract
Objective: To study the effects of electro-acupuncture with low frequency current on expressions of N-methyl-D-aspartate(NMDA) receptor subtypes (NR1, NR2A, NR2B) in hippocampus of heroin psychological dependent mice, and to investigate the possible neuromechanism of heroin addictive memory intervention in experimental mice.
Method: Forty-five mice without natural place preference were equally and randomly divided into: control group, model group, model with electro-acupuncture (EA) therapy group（15mice/group）. The experimental heroin psychological dependent models were established by heroin injection with progressively increasing dose and conditioned place preference(CPP) in mice. Electro-acupuncture with low frequency current of 2Hz needling on “Neiguan(PC6)”, “Shanyinjiao(SP6)”, “Sishenchong(EX-HN1)” points. The behaviour index was measured by CPP, and the expression of NMDA receptor subtype - in hippocampus were detected by immunohistochemistry.
Result: ①The mice of EA group stayed longer in heroin-paired chamber than that of model group (P＜0.05). ②Comparing with model group, the expressions of NR1 and NR2B in hippocampus increased (P＜0.05). ③The expression of NR2A in hippocampus reduced (P＜0.05).
Conclusion: EA could increase the expressions of NR1, NR2B and reduce the expression of NR2A. The possible neuromechanism of these changes might be related to heroin addictive memory of experimental mice treated by electro-acupuncture with low frequency current. |
| Keywords:heroin dependence hippocampus electro-acupuncture with low frequency current N-Methyl-D-aspartate receptor subtype |
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