| 毕迎立,范建中,陈银海.红外偏振光对骨髓间充质干细胞的影响[J].中国康复医学杂志,2012,27(12):1093~1096 |
| 红外偏振光对骨髓间充质干细胞的影响 点此下载全文 |
| 毕迎立 范建中 陈银海 |
| 南方医科大学南方医院康复医学科，广州，510515 |
| 基金项目：广东省自然科学基金项目（8451051501000460）；广州市海珠区科技计划项目（2009-Y-018） |
| DOI： |
| 摘要点击次数: 1771 |
| 全文下载次数: 2094 |
| 摘要： |
| 摘要
目的：观察红外偏振光治疗仪的临床治疗剂量对骨髓间充质干细胞（BMSCs）生长情况的影响。
方法：采用全骨髓培养法获取SD大鼠的原代BMSCs，取传代培养的细胞分为：实验组：红外偏振光处理10min；对照组：空气暴露相同时间。采用CCK8法检测细胞的增殖活性，流式细胞术进行细胞凋亡检测和细胞周期分析。
结果：CCK8法检测结果显示，实验组的光密度（OD）值与对照组相比，差异无显著性意义（P＞0.05）；细胞凋亡检测显示，两组的结果差异无显著性意义（P＞0.05）；细胞周期分析结果显示，两组差异无显著性意义（P＞0.05）。
结论：红外偏振光的临床治疗剂量对骨髓间充质干细胞的增殖无明显影响，没有发现引起细胞凋亡，对细胞周期无明显影响。 |
| 关键词：红外偏振光 骨髓间充质干细胞 增殖 凋亡 细胞周期 |
| The effect of linear polarized near-infrared on bone marrow stromal stem cells Download Fulltext |
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| Rehabilitation Medicine Department, Nanfang Hospital of Southern Medical University, Guangzhou, 510515 |
| Fund Project: |
| Abstract: |
| Abstract
Objective: To investigate the effect of linear polarized near-infrared on the growth of bone marrow stromal stem cells (BMSCs).
Method: The primary BMSCs were got from Sprague-Dawley rats with the method of whole bone marrow adherent cultivation. The passage cells were divided into trial group with linear polarized near-infrared irradiation for 10min and control group with disposing for the same time without linear polarized near-infrared irradiation. The condition of cell proliferation was measured using CCK8 method. The cell apoptosis and cell cycle were analysed with flow cytometry (FACS).
Result: CCK8 assay revealed no significant change of optical density（OD） in proliferation of the cells irradiated by linear polarized near-infrared(P＞0.05). Flow cytometry results had no significant variation of cell apoptosis (P＞0.05), nor had significant variation of cell cycle (P＞0.05).
Conclusion: The linear polarized near-infrared with clinical treatment dosage has no effect on the proliferation, apoptosis and cell cycle of BMSCs. |
| Keywords:linear polarized near-infrared bone marrow stromal stem cell proliferation apoptosis cell cycle |
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