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金 玲,黄民江,李洪亮,江兴林,曹湘玉.神经节苷脂对实验性颅脑外伤大鼠的保护作用及机制[J].中国康复医学杂志,2013,28(12):1142~1145
神经节苷脂对实验性颅脑外伤大鼠的保护作用及机制    点此下载全文
金 玲  黄民江  李洪亮  江兴林  曹湘玉
怀化医学高等专科学校,湖南怀化,418000
基金项目:湖南省科技厅科技计划项目(2009FJ3200);湖南省中医药管理局科研计划项目(2012124)
DOI:
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全文下载次数: 1934
摘要:
      摘要 目的:通过观察神经节苷脂(GM1)对实验性颅脑外伤大鼠大脑海马区神经巢蛋白(nestin)、神经生长因子(NGF)表达的影响,探讨GM1促进脑神经修复的可能机制。 方法:选择出生7d雄性SD大鼠180只,随机分为三组:空白组(假手术组)60只,无颅脑外伤;对照组60只(颅脑外伤,腹腔注射生理盐水);实验组60只(颅脑外伤,腹腔注射GM1)。分别在术后第0、2、4、8、12、16、24小时不同时间点利用免疫组化方法检测大鼠大脑海马区nestin、NGF表达情况和整个实验过程中颅脑外伤动物模型造模情况。 结果:①实验组颅脑外伤造模后自然死亡率10%,明显低于对照组的25%(P<0.05);②实验组大鼠大脑海马区的nestin 第0—24小时表达与对照组比较无显著差异(P>0.05),实验组和对照组大鼠大脑海马区的nestin 第0—24小时表达明显高于空白组,差异有显著性(P<0.05);③实验组大鼠大脑海马区NGF的表达均明显高于对照组和空白组,差异有非常显著性(P<0.01),另外实验组大脑海马区NGF的表达随着时间的增加而调低,至第24小时接近空白组水平但仍高于对照组水平。 结论:预防性地使用GM1不仅可使颅脑外伤模型大鼠大脑海马区NGF表达明显增高,同时能降低颅脑外伤造模大鼠的死亡率,这可能是通过GM1提高实验性颅脑外伤大鼠的NGF表达并延长表达时间来实现的。
关键词:颅脑外伤  神经节苷脂  巢蛋白  神经生长因子  大鼠
The protection effect of ganglion monoglyceride on experimental craniocerebral trauma rats and its mechanism    Download Fulltext
Huaihua Medical College, Hunan Province,418000
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Abstract:
      Abstract Objective: To observe the effect of ganglion monoglyceride (GM1) on experimental craniocerebral trauma rats' hippocampus nestin and nerve growth factor (NGF) expressions, and to explore the possible mechanisms of GM1 promoting nerve repairing. Method: Of birth 7 days 180 male SD rats were randomly divided into three groups. Blank group (sham group) 60, no craniocerebral trauma. Craniocerebral trauma model 120, were divided into control group 60 (intraperitoneal injection of saline), the experimental group 60 (intraperitoneal injection of GM1). Respectively, at the postoperation at the 0h, 2nd h, 4th h, 8th h, 12th h, 16th h, 24th h different time points rat hippocampus nestin, as well as NGF expressions and the whole experimental process of rats craniocerebral trauma modeling were detected by immunohistochemistry. Result: ①In experimental group after craniocerebral trauma modeling the rats' natural mortality was 10%, it was significantly lower than the rate 25% in control group (P<0.05). ②In experimental rats' hippocampus expressions of nestin at the 0—24h compared with that in control group had no significant difference (P>0.05). But the expressions of nestin at the 0—24h in experimental and control rats hippocampus were significantly higher than that in blank group, there were significant differences (P<0.05). ③Experimental rats' hippocampus had significant higher expression of NGF than that in control and blank group(P<0.01); In the hippocampus of experimental group expression of NGF down-regulated with the time lengthening and at the 24h closed to the level of blank group but was still higher than the level of control group. Conclusion: GM1 could not only significantly increased craniocerebral trauma rats' hippocampus NGF expression, while reduced mortality of model rats which may be achieved by GM1 increased and extended the expression of NGF of experimental rats.
Keywords:craniocerebral trauma  ganglion monoglyceride  nestin  nerve growth factor  rat
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