| 潘 璠,于天源,吴剑聪,高玉峰,鲁梦倩,李小琴,于 跃,耿 楠,冼思彤,张林峰.电针对坐骨神经损伤大鼠背根神经节中神经营养因子-3及其受体TrkC的影响[J].中国康复医学杂志,2014,29(12):1109~1112 |
| 电针对坐骨神经损伤大鼠背根神经节中神经营养因子-3及其受体TrkC的影响 点此下载全文 |
| 潘 璠 于天源 吴剑聪 高玉峰 鲁梦倩 李小琴 于 跃 耿 楠 冼思彤 张林峰 |
| 北京中医药大学针灸推拿学院，北京，100029 |
| 基金项目：国家自然科学基金资助项目（81373759）；博士点专项科研基金资助项目（20130013110016）；北京市自然科学基金资助项目（7142097） |
| DOI： |
| 摘要点击次数: 1579 |
| 全文下载次数: 1867 |
| 摘要： |
| 摘要
目的：观察电针对坐骨神经损伤大鼠行为学、神经生长因子-3及其受体TrkC 的影响，探讨电针治疗坐骨神经损伤的生物学机制。
方法：建立大鼠坐骨神经夹持损伤模型，观察各组大鼠的行为学改变与背根神经节（DRG）中NT-3与TrkC表达情况。
结果：模型组、模型对照组的大鼠行为学检测表明，造模后大鼠的感觉功能显著下降（P＜0.05），电针治疗后有显著改善（P＜0.05），与正常组无显著差异；电针组的NT-3与TrkC表达显著升高（P＜0.05），第20天时模型组与正常组相比，NT-3分泌显著增多（P＜0.05）。
结论：电针治疗可以通过提高NT-3与TrkC的表达，维持神经元存活，促进受损神经修复，改善坐骨神经损伤大鼠的感觉功能。 |
| 关键词：电针 坐骨神经损伤 神经营养因子-3 酪氨酸激酶受体 |
| Investigation of electroacupuncture on NT-3 and TrkC expression in dorsal root ganglia in rats with sciatic nerve injury Download Fulltext |
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| School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing,100029 |
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| Abstract: |
| Abstract
Objective: To observe the influence of electroacupuncture on ethology, expressions of neurotrophic factor-3（NT-3） and tyrosine kinase receptor type C（TrkC） receptor of NT-3 in dorsal root ganglia(DRG) of rats with sciatic nerve injury, and to investigate the biological mechanisms of electroacupuncture in sciatic nerve injury.
Method: Sciatic nerve clamping injury were used as the model. The thermal withdraw latency tests were operated to observe the behavior changes. The level of NT-3 and TrkC in DRG was measured by immunohistochemistry.
Result: The sensory function of model group and model control group reduced significantly(P＜0.05). The score of thermal withdraw latency(PWL) increased significantly after electroacupuncture therapy（P＜0.05）The expressions of NT-3 and TrkC in electroacupuncture group increased significantly compared with other four groups (P＜0.05). On the 20th d the expression of NT-3 in model group increased significantly compared with normal group (P＜0.05).
Conclusion: Electroacupuncture could promote neuron survival, stimulate the recovery of injured nerve and improve sensory function by increasing the release of NT-3 and TrkC in rats with sciatic nerve injury. |
| Keywords:electroacupuncture sciatic nerve injury neurotrophic factor-3 tyrosine kinase receptor type C |
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