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何任红,范建中,李德洁.低声压级次声对脑缺血再灌注大鼠的突触素和微管相关蛋白表达的影响[J].中国康复医学杂志,2015,(1):10~13
低声压级次声对脑缺血再灌注大鼠的突触素和微管相关蛋白表达的影响    点此下载全文
何任红  范建中  李德洁
南方医科大学南方医院康复医学科,广州,510515
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DOI:
摘要点击次数: 1938
全文下载次数: 2023
摘要:
      摘要 目的:探讨低声压级次声对脑缺血再灌注损伤大鼠治疗后梗死灶周围组织的突触素(SYN)和微管相关蛋白(MAP-2)表达的影响。 方法:线栓法制作脑缺血再灌注模型,18只SD大鼠随机分为假手术组、模型组、次声组,每组6只。假手术组大鼠大脑中动脉没有栓塞且不进行次声干预,模型组大鼠造模后不行次声干预,次声组大鼠造模成功12h后连续次声干预7d,每天2h。在第8天对大鼠用改良神经功能损害评分法(mNSS)进行神经功能评分,然后心脏灌注取脑、固定、切片进行免疫组化检测SYN和MAP-2。 结果:次声组的大鼠神经功能mNSS评分比模型组明显降低,次声组梗死灶周围组织SYN的累计光密度(IOD)比模型组显著增高(P<0.01);次声组梗死灶周围组织MAP-2的染色比模型组强(P<0.01)。 结论:低声压级次声能促进脑缺血再灌注大鼠功能恢复的机制之一可能是促进了梗死灶周围组织内的SYN和MAP-2的表达,提高了神经元的可塑性。
关键词:次声  脑缺血再灌注  突触素  微管相关蛋白2
Effects of infrasound of low sound-pressure on the expressions of synapsin and microtubule associated protein-2 in cerebral ischemia-reperfusion rats    Download Fulltext
Department of Rehabilitation Medicine, Nanfang Hospital of Southern Medical University, Guangzhou, 510515
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Abstract:
      Abstract Objective: To explore effects of infrasound of low sound pressure on the expressions of synapsin I (SYN) and microtubule associated protein-2 (MAP-2) in cerebral ischemia-reperfusion rats. Method: Rats of infrasound group (n=6) and model group (n=6) were conducted middle cerebral artery occlusion by thread embolism; the sham (n=6) group hadn't embolism and wasn't treated with infrasound. Rats in infrasound group were treated with infrasound for two hours per day. The neural function scores of each group were scored by using modified neurological severity scores(mNSS) at the 8th day. Then, the rats were executed and brain tissue slices were stained to observe the expression of SYN and MAP-2 around the ischemic sites by immunohistochemical technique. Result: The mNSS score of infrasound group was higher than that of model group (P<0.05). The expression of SYN in infrasound group was significantly higher than that in model group (P<0.01). The expression intensity of MAP-2 in infrasound group was stronger than that in model group(P<0.01). Conclusion: One of the mechanisms in response to the therapeutic effects of infrasound of low sound-pressure on cerebral ischemia-reperfusion rats may be the improvement of expressions of SYN and MAP-2.
Keywords:infrasound  cerebral ischemia-reperfusion  synapsin  microtubule associated protein-2
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