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李灵玲,周达岸,杨燕兴,王 良,刘鹏民.联合应用督脉电针和游泳训练后脊髓损伤大鼠神经干细胞分化方向的研究[J].中国康复医学杂志,2016,(2):172~176
联合应用督脉电针和游泳训练后脊髓损伤大鼠神经干细胞分化方向的研究    点此下载全文
李灵玲  周达岸  杨燕兴  王 良  刘鹏民
辽宁医学院,锦州,121001
基金项目:辽宁医学院校长基金——奥鸿博泽研究生科研创新基金
DOI:
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摘要:
      摘要 目的:联合应用督脉电针和游泳训练后,研究脊髓损伤大鼠神经干细胞分化的方向。 方法:复制并评价脊髓全横断损伤大鼠模型,75只大鼠随机分为5组:假手术组、脊髓损伤组、脊髓损伤+督脉电针组、脊髓损伤+游泳训练组、脊髓损伤+督脉电针+游泳训练组(n=15),检测各组1周、2周、3周、4周、5周5个时间点脊髓组织神经生长因子(NGF)、胶质纤维酸性蛋白(GFAP)的表达,对各组大鼠进行BBB评分(Basso, Beattie & Bresnahan locomotor rating scale, BBB scale)。 结果:各治疗组均能使不同时间点脊髓损伤大鼠BBB评分、脊髓组织NGF表达水平提高(P<0.05);脊髓组织GFAP表达水平下降(P<0.05);提示游泳训练和督脉电针联合干预对促进脊髓损伤的康复效果更佳。各治疗组随着干预时间的增加表现出不同程度的BBB评分、脊髓组织NGF表达水平提高(P<0.01),脊髓组织GFAP表达水平下降(P<0.01);尽量长时间的应用游泳训练和督脉电针对促进脊髓损伤大鼠运动功能恢复的疗效最佳。 结论:联合应用督脉电针与游泳训练后,神经干细胞分化方向得到控制,使脊髓组织NGF表达增强,GFAP表达被抑制,从而促进神经元的再生和修复,抑制星形胶质细胞持续反应性增生,减少胶质瘢痕组织生成,促进神经环路重建。
关键词:神经干细胞  督脉电针  游泳训练  脊髓损伤  神经环路
Effects of Du Mai electroacupuncture combined with swim training on differentiation of neural stem cell after spinal cord injury in rats    Download Fulltext
Liaoning Medical College, Jinzhou, 121001
Fund Project:
Abstract:
      Abstract Objective: To investigate the effect of Du Mai electroacupuncture and Swim Training on differentiation of neural stem cell after spinal cord injury in rats. Method: Copy and evaluate rat model after spinal cord injury (SCI). The rats were randomly divided into five groups: sham operation group, SCI group, SCI and Du Mai electroacupuncture group (SCI+EA), SCI and Swim Training group (SCI+ST), SCI with Du Mai electroacupuncture and Swim Training group (SCI+DA+ST). Detected the expression of nerve growth factor (NGF), glial fibrillary acidic protein (GFAP) at 1w, 2w, 3w, 4w, 5w five time points. Result: The scores of BBB scales, levels of NGF increased (P<0.05) and the expression of GFAP depressed (P<0.05) in treatment groups at different time points respectively. The combination therapy of ST and DE was better in promoting the recovery of motor function for SCI rats. With the intervention time change, the treatment groups show different degrees of BBB score, expression of NGF increased (P<0.01) and expression of GFAP decreased (P<0.01). Extend the application of ST and ED on SCI rats to recovery of motor function is the best curative effect. Conclusion: The combination therapy of ST and EA can control the differentiation of neural stem cell, enhance the expression of NGF and inhibit the expression of GFAP, so as to promote neuronal regeneration and repair, prevent sustained reactive hyperplasia of astrocyte cells, reduce glial scar tissue, thus promote neural circuit reconstruction.
Keywords:neural stem cells  acupuncture  swimming training  spinal cord injury  neural circuits
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