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冯杰扬,陈杨葭,郭 磊,钟剑锋,张桂通,刘 佳,叶超群.减重步行训练对不完全脊髓损伤大鼠脚桥核可塑性影响的研究[J].中国康复医学杂志,2017,(6):624~630
减重步行训练对不完全脊髓损伤大鼠脚桥核可塑性影响的研究    点此下载全文
冯杰扬  陈杨葭  郭 磊  钟剑锋  张桂通  刘 佳  叶超群
南方医科大学第二临床医学院,广东,510515
基金项目:国家自然科学基金应急管理项目(81650013);国家自然科学基金面上项目(81171862)
DOI:
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摘要:
      摘要 目的:探索减重步行训练对不完全脊髓损伤大鼠脚桥核可塑性的影响。 方法:将雌性SD大鼠24只分为减重步行训练组、未训练组和假手术组,每组8只,用美国NYU脊髓冲击损伤仪制作大鼠T10脊髓不完全损伤模型。采用PET-CT、免疫荧光染色及BBB评分观察减重步行训练对不完全脊髓损伤(iSCI)大鼠中脑运动区(MLR)脚桥核可塑性变化和后肢运动功能的改善。 结果:减重步行训练组大鼠的后肢运动功能BBB评分在造模后4周、7周两个时间点的分值均较未训练组高(P<0.05)。假手术组大鼠在造模后1周、4周、7周3个时间点的BBB评分值均明显高于训练组和未训练组(P<0.01)。虽然训练组18F-氟脱氧葡萄糖(18F-FDG)的标准摄取值(SUV)的平均值较未训练组增高,而且假手术组的SUV值最高,但三组SUV值的差异不具有显著性意义(P>0.05)。神经元特异性核抗原(NeuN)免疫荧光染色检测显示训练组、未训练组、假手术组三组间大鼠脚桥核的神经元数量差异无显著性意义(P>0.05),而脚桥核2型囊泡谷氨酸转运体(VGLUT2)的积分光密度值(IOD)比较,假手术组IOD表达最高,训练组较未训练组明显增强(P<0.01)。 结论:减重步行训练能够明显提高iSCI大鼠后肢运动功能及其脚桥核中谷氨酸能信号传递,而且iSCI后大鼠中脑脚桥核的整体代谢及神经元数量无明显改变。因此认为脚桥核的谷氨酸表达增强是减重步行训练使iSCI大鼠后肢步行功能改善的原因之一。减重步行训练能使脚桥核发生神经递质表达层面的可塑性变化。
关键词:不完全性脊髓损伤  减重步行训练  脚桥核  2型囊泡谷氨酸转运体
Research on the plasticity of pedunculopontine tegmental nucleus after body weight supported treadmill training in incomplete spinal cord injury rats    Download Fulltext
The Second Clinical Medicine College, Southern Medical University, Guangdong, 510515
Fund Project:
Abstract:
      Abstract Objective: To investigate the plasticity of pedunculopontine tegmental nucleus according to the changes of standardized uptake value(SUV) and vessicular glutamate transporters 2(VGLUT2) expression after body weight support treadmill training (BWSTT) in incompletely spinal cord injury rats. Method: Twenty-four Sprague-Dawley rats were randomly divided into the sham operated group (n=8), the untrained control group (n=8), and BWSTT treatment group (n=8). Spinal cord moderate contusion model was made at T10 segment with NYU impactor. Behavioral ability was evaluated by Basso-Beattie-Bresnahan (BBB) scale of hind limb movement. The 18F-FDG SUV of PPTg was collected by PET/CT. Furthermore, the VGLUT2 expression in PPTg were processed by immunofluorescence for quantitative analysis. Result: Comparing with the control group,BBB scores increased significantly more in the BWSTT treatment group (P<0.05) at 7 weeks post-injury, whereas scores in BWSTT treatment group and control group are still much lower than those in sham group (P<0.05). The expression of VGLUT2 in BWSTT treatment group is much higher than that in untrained control group, and sham operated group is the highest among three groups. Conclusion: BWSTT can partially improve recovery of hind limbs locomotion. One of the mechanism is that it markedly promote neurohumoralplasticity of PPTg in rat after iSCI
Keywords:incomplete spinal cord injury  body weight support treadmill training  pedunculopontine tegmental nucleus  vessicular glutamate transporters 2
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