| 李晓洁,俞坤强,赵从快,彭洪卫,李春燕,卓沛元,林如辉,陶 静,陈立典.电针神庭、百会对大脑中动脉闭塞大鼠学习记忆的影响及机制研究[J].中国康复医学杂志,2017,(8):869~873 |
| 电针神庭、百会对大脑中动脉闭塞大鼠学习记忆的影响及机制研究 点此下载全文 |
| 李晓洁 俞坤强 赵从快 彭洪卫 李春燕 卓沛元 林如辉 陶 静 陈立典 |
| 福建中医药大学康复医学院，福州，350122 |
| 基金项目：福建省自然科学基金项目（2015J01335）；福建省康复技术协同创新中心资助项目（X2012004） |
| DOI： |
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| 摘要
目的：研究磷酸化的环磷腺苷效应元件结合蛋白（p-cAMP-response element binding protein,p-CREB）、脑源性神经营养因子（brain-derived neurotrophic factor, BDNF）在大鼠脑缺血再灌注中的表达，探讨电针神庭、百会对脑缺血再灌注大鼠学习记忆功能的影响及其可能机制。
方法：36只雄性SD大鼠，随机分为假手术组、模型组和电针组，每组分配12只。采用大脑中动脉闭塞（middle cerebral artery occlusion，MCAO）法建立局灶性脑缺血再灌注大鼠模型。电针组电针神庭、百会穴干预7d。各组采用Morris水迷宫实验评估大鼠的学习记忆功能；TTC染色观察缺血梗死体积；免疫组化染色检测海马CA1区p-CREB，BDNF的表达。
结果：与假手术组比较，模型组鼠到达水下平台的潜伏期长些(P<0.01)，通过平板的次数少些(P<0.01)。电针组与模型组比较潜伏期显著短些(P<0.05)，通过平台的次数显著多些(P<0.01)。与假手术组比较，模型组的 p-CREB阳性细胞明显减少而BDNF光密度明显增加。与模型组比较，电针组的p-CREB阳性细胞明显增加(P<0.01)而BDNF光密度也增加(P<0.05)，梗死的体积则小些。
结论：电针神庭、百会可改善脑缺血再灌注大鼠的学习记忆功能，其作用机制可能与上调缺血侧海马CA1区p-CREB，BDNF的表达有关。 |
| 关键词：电针 脑缺血 学习记忆 磷酸化的环磷腺苷效应元件结合蛋白 脑源性神经营养因子 |
| The effects and mechanisms of electroacupuncture at Shenting and Baihui acupoints on learning and memory in MCAO rats Download Fulltext |
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| College of Rehabilitation Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, 350122 |
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| Abstract: |
| Abstract
Objective: To investigate p-cAMP-response element binding protein（p-CREB）, brain-derived neurotrophic factor（BDNF） expressions in rats after focal cerebral ischemia-reperfusion of rats, and electroacupuncture at Shenting and Baihui acupoints.
Method: SD rats (n=36) were randomly divided into sham operation control group (Sham group), model group (I/R group) and electroacupuncture group (I/R+EA group). Each group had 12 rats. Middle cerebral artery occlusion (MCAO) was used to set up the focal cerebral I/R injury for I/R rats. The rats in I/R+EA group received electroacupuncture treatment at Shenting (DU24) and Baihui (DU20) for 7 days. Morris water maze was used to investigate learning and memory ability daily. TTC staining was used to assess the cerebral infarct volume. Immunohistochemistry was performed to test the expressions of p-CREB and BDNF in the hippocampus CA1 zone of the cerebral ischem(P<0.01) (P<0.01)ia side.
Result: The rats in the model group demonstrated longer latency to reach the hidden platform(P<0.01) and passed the platform position fewer in the water maze test(P<0.01) than the sham group. In the electroacupuncture group, the rats significantly shorten the latency (P<0.05) and increased the times of passing the platform(P<0.01) compared with the model group. Compared with the sham group, the number of positive cells of p-CREB in model group was significantly reduced (P<0.01), the mean optical density of BDNF was significantly higher (P<0.01); Compared with the model group, the number of positive cells of p-CREB in electroacupuncture group was significantly increased (P<0.01), the mean optical density of BDNF was higher (P<0.05), the infarct volume was decreased (P<0.01).
Conclusion: Electroacupuncture can ameliorate learning and memory ability of cerebral ischemia-reperfusion rats. The mechanism may be related to increase the expressions of p-CREB and BDNF in hippocampus CA1 zone. |
| Keywords:electroacupuncture cerebral ischemia learning and memory p-cAMP-response element binding protein brain-derived neurotrophic factor |
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