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李晓洁,俞坤强,赵从快,彭洪卫,李春燕,卓沛元,林如辉,陶 静,陈立典.电针神庭、百会对大脑中动脉闭塞大鼠学习记忆的影响及机制研究[J].中国康复医学杂志,2017,(8):869~873
电针神庭、百会对大脑中动脉闭塞大鼠学习记忆的影响及机制研究    点此下载全文
李晓洁  俞坤强  赵从快  彭洪卫  李春燕  卓沛元  林如辉  陶 静  陈立典
福建中医药大学康复医学院,福州,350122
基金项目:福建省自然科学基金项目(2015J01335);福建省康复技术协同创新中心资助项目(X2012004)
DOI:
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摘要:
      摘要 目的:研究磷酸化的环磷腺苷效应元件结合蛋白(p-cAMP-response element binding protein,p-CREB)、脑源性神经营养因子(brain-derived neurotrophic factor, BDNF)在大鼠脑缺血再灌注中的表达,探讨电针神庭、百会对脑缺血再灌注大鼠学习记忆功能的影响及其可能机制。 方法:36只雄性SD大鼠,随机分为假手术组、模型组和电针组,每组分配12只。采用大脑中动脉闭塞(middle cerebral artery occlusion,MCAO)法建立局灶性脑缺血再灌注大鼠模型。电针组电针神庭、百会穴干预7d。各组采用Morris水迷宫实验评估大鼠的学习记忆功能;TTC染色观察缺血梗死体积;免疫组化染色检测海马CA1区p-CREB,BDNF的表达。 结果:与假手术组比较,模型组鼠到达水下平台的潜伏期长些(P<0.01),通过平板的次数少些(P<0.01)。电针组与模型组比较潜伏期显著短些(P<0.05),通过平台的次数显著多些(P<0.01)。与假手术组比较,模型组的 p-CREB阳性细胞明显减少而BDNF光密度明显增加。与模型组比较,电针组的p-CREB阳性细胞明显增加(P<0.01)而BDNF光密度也增加(P<0.05),梗死的体积则小些。 结论:电针神庭、百会可改善脑缺血再灌注大鼠的学习记忆功能,其作用机制可能与上调缺血侧海马CA1区p-CREB,BDNF的表达有关。
关键词:电针  脑缺血  学习记忆  磷酸化的环磷腺苷效应元件结合蛋白  脑源性神经营养因子
The effects and mechanisms of electroacupuncture at Shenting and Baihui acupoints on learning and memory in MCAO rats    Download Fulltext
College of Rehabilitation Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, 350122
Fund Project:
Abstract:
      Abstract Objective: To investigate p-cAMP-response element binding protein(p-CREB), brain-derived neurotrophic factor(BDNF) expressions in rats after focal cerebral ischemia-reperfusion of rats, and electroacupuncture at Shenting and Baihui acupoints. Method: SD rats (n=36) were randomly divided into sham operation control group (Sham group), model group (I/R group) and electroacupuncture group (I/R+EA group). Each group had 12 rats. Middle cerebral artery occlusion (MCAO) was used to set up the focal cerebral I/R injury for I/R rats. The rats in I/R+EA group received electroacupuncture treatment at Shenting (DU24) and Baihui (DU20) for 7 days. Morris water maze was used to investigate learning and memory ability daily. TTC staining was used to assess the cerebral infarct volume. Immunohistochemistry was performed to test the expressions of p-CREB and BDNF in the hippocampus CA1 zone of the cerebral ischem(P<0.01) (P<0.01)ia side. Result: The rats in the model group demonstrated longer latency to reach the hidden platform(P<0.01) and passed the platform position fewer in the water maze test(P<0.01) than the sham group. In the electroacupuncture group, the rats significantly shorten the latency (P<0.05) and increased the times of passing the platform(P<0.01) compared with the model group. Compared with the sham group, the number of positive cells of p-CREB in model group was significantly reduced (P<0.01), the mean optical density of BDNF was significantly higher (P<0.01); Compared with the model group, the number of positive cells of p-CREB in electroacupuncture group was significantly increased (P<0.01), the mean optical density of BDNF was higher (P<0.05), the infarct volume was decreased (P<0.01). Conclusion: Electroacupuncture can ameliorate learning and memory ability of cerebral ischemia-reperfusion rats. The mechanism may be related to increase the expressions of p-CREB and BDNF in hippocampus CA1 zone.
Keywords:electroacupuncture  cerebral ischemia  learning and memory  p-cAMP-response element binding protein  brain-derived neurotrophic factor
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